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Publication
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| Title of Article |
Development and Efficacy of Droplet Digital PCR for Detection of Strongyloides
stercoralis in Stool |
| Date of Acceptance |
1 September 2021 |
| Journal |
| Title of Journal |
The American Journal of Tropical Medicine and Hygiene |
| Standard |
SCOPUS |
| Institute of Journal |
American journal of tropical medicine and hygiene |
| ISBN/ISSN |
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| Volume |
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| Issue |
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| Month |
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| Year of Publication |
2022 |
| Page |
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| Abstract |
Human strongyloidiasis is one of the neglected tropical diseases caused by infection with soil-transmitted
helminth Strongyloides stercoralis. Conventional stool examination, a method commonly used for diagnosis of S. stercoralis, has low sensitivity, especially in the case of light infections. Herein, we developed the droplet digital polymerase
chain reaction (ddPCR) assay to detect S. stercoralis larvae in stool and compared its performance with real-time PCR
and stool examination techniques (formalin ethyl-acetate concentration technique [FECT] and agar plate culture [APC]).
The ddPCR results showed 98% sensitivity and 90% specificity, and real-time PCR showed 82% sensitivity and 76.7%
specificity when compared with the microscopic methods. Moreover, ddPCR could detect a single S. stercoralis larva in
feces, and cross-reactions with other parasites were not observed. In conclusion, a novel ddPCR method exhibited high
sensitivity and specificity for detection of S. stercoralis in stool samples. This technique may help to improve diagnosis,
particularly in cases with light infection. In addition, ddPCR technique might be useful for screening patients before starting immunosuppressive drug therapy, and follow-up after treatment of strongyloidiasis. |
| Keyword |
Strongyloides stercoralis, droplet digital polymerase chain reaction (ddPCR), molecular technique, Diagnosis |
| Author |
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| Reviewing Status |
มีผู้ประเมินอิสระ |
| Status |
ตีพิมพ์แล้ว |
| Level of Publication |
นานาชาติ |
| citation |
true |
| Part of thesis |
true |
| Attach file |
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| Citation |
0
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Journal Publication
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