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Publication
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| Title of Article |
Development of a Highly Efficient Callus Induction and
Regeneration Protocol for Sugarcane using Apical Meristem |
| Date of Acceptance |
11 February 2022 |
| Journal |
| Title of Journal |
Development of a Highly Efficient Callus Induction and Regeneration Protocol for Sugarcane using Apical Meristem |
| Standard |
SCOPUS |
| Institute of Journal |
Asian Journal of Plant Sciences |
| ISBN/ISSN |
- |
| Volume |
21 |
| Issue |
1 |
| Month |
ตุลาคม |
| Year of Publication |
2022 |
| Page |
78-87 |
| Abstract |
Background and Objective: Sterilized plants derived from apical meristem culture are proposed to overcome some disease problems
in sugarcane. However, the success rate of this technique depends upon cultural media compositions. Thus, this study aimed to determine
the optimum concentration of 2,4-D and light conditions on callus development derived from the apical meristem and to stimulate the
development of sugarcane shoots and roots. Materials and Methods: The apical meristem of the KK3 genotype was used. A 5×2 factorial
in Completely Randomized Design (CRD) with 20 replications was assigned. Five concentrations of 2,4-D namely 0, 1, 2, 3 and 4 mg LG1
were assigned as factor A, while light and dark conditions were assigned as factor B. For a further investigation on the shoot and root
regenerations, four concentrations of coconut water namely 0, 10, 20 and 30% (v/v) were applied in the MS media and were assigned in
CRD with 20 replications. Results: MS media supplemented with 2 mg LG1
2,4-D under dark conditions could generate the highest callus
induction rate (100%), big callus sizes (0.72 cm) and a good callus weight (65.75 mg). A 10% of coconut water was the optimum
concentration, yielding the most prolific shoots per callus (25 shoots), the longest shoots (4 cm) and the most prolific roots per callus
(43 roots). Conclusion: Application of 2 mg LG1
2,4-D under dark conditions provided the most effective callus induction of sugarcane and
10% (v/v) coconut water could generate the most prolific shoots and roots per callus. |
| Keyword |
Sugarcane seedling, callus induction, plantlet regeneration, coconut water, callus size |
| Author |
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| Reviewing Status |
ไม่มีผู้ประเมินอิสระ |
| Status |
ตีพิมพ์แล้ว |
| Level of Publication |
นานาชาติ |
| citation |
false |
| Part of thesis |
true |
| Attach file |
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| Citation |
0
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Journal Publication
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