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Publication
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| Title of Article |
Potential of Anethum graveolens L . to up-regulate the expression of phase I and II metabolizing genes in HepG2 cells |
| Date of Acceptance |
29 November 2021 |
| Journal |
| Title of Journal |
Tropical Journal of Pharmaceutical Research |
| Standard |
ISI |
| Institute of Journal |
Pharmacotherapy Group, Faculty of Pharmacy, University of Benin, Benin City, 300001 Nigeria. |
| ISBN/ISSN |
1596-5996 (print); 1596-9827 (electronic) |
| Volume |
2021 |
| Issue |
20(12) |
| Month |
December |
| Year of Publication |
2022 |
| Page |
2519-2525 |
| Abstract |
Purpose: To investigate the effects of the herbal medicine Anethum graveolens L. (AG) on the expressions of phase I and II metabolizing genes in HepG2 cells in order to gain insight into the metabolism of AG. Methods: HepG2 cells (5 x 105 cells/well) were treated with either 10 µM ketoconazole, 20 µM rifampicin, or AG extract (60 - 480 µg/mL) for 72 h. Cell viability and reactive oxygen species (ROS) production were assessed using resazurin and 2′,7′-dichlorofluorescein diacetate assays, respectively. The mRNA expression of phase I (CYP1A2, CYP2C19, CYP2D6, and CYP3A4) and phase II metabolizing enzymes (UGT1A6 and NAT1) were determined using reverse transcription-real-time polymerase chain reaction (qRT-PCR). Results: ROS production was not affected by the various treatments. The highest concentration of AG (480 µg/mL) reduced cell viability to 63.33 %. The expressions of CYP1A2, CYP2C19, CYP2D6, and NAT1 mRNA were significantly elevated after 72-h AG treatment. Conclusion: The use of AG as an alternative medicine, particularly at high concentrations and/or after prolonged use, poses a risk for herb-drug interactions due to the up-regulation of CYP1A2, CYP2C19, CYP2D6, and NAT1 expression by AG in HepG2 cells. |
| Keyword |
Dill, Reactive oxygen species, Metabolizing enzymes, Drug interaction, Hepatocellular carcinoma cell |
| Author |
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| Reviewing Status |
มีผู้ประเมินอิสระ |
| Status |
ตีพิมพ์แล้ว |
| Level of Publication |
นานาชาติ |
| citation |
true |
| Part of thesis |
true |
| Attach file |
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| Citation |
0
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Journal Publication
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