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Journal Publication
Title of Article The Melatonin Treatment Improves the Ovarian Responses After Superstimulation in Thai-Holstein Crossbreds under Heat Stress Conditions 
Date of Acceptance 1 April 2022 
Journal
     Title of Journal Frontiers in Veterinary Science  
     Standard SCOPUS 
     Institute of Journal Frontiers Media S.A. 
     ISBN/ISSN  
     Volume  
     Issue  
     Month
     Year of Publication 2022 
     Page  
     Abstract The effect of heat stress with melatonin treatment on the superovulatory responses and embryo characteristics in Thai-Holstein crossbreds under heat stress conditions was examined. Six non-lactating cows (replication = 4; n = 24) were assigned to one of 2 treatments in double cross-over design. All cows were superstimulated with traditional treatment. Melatonin group (n=12): cows received intramuscularly injection 18 mg./50 kg. simultaneously with GnRH injection, while those in the control group (n=12) received none. Bloods samples were taken to determine lipid peroxidation (MDA) and the activity of the antioxidant enzymes (superoxide dismutase; SOD). The experiment was conducted from April to September, which determined severe heat stress (the mean temperature-humidity index above 77). The results revealed that numbers of large follicles and corpora lutea were higher in the melatonin group than in the control group (p<0.01). Numbers of recovered ova/embryos, fertilized ova, and transferable embryos were higher in the melatonin group (p<0.01); meanwhile, more degenerated embryos were found in the control group (p<0.01). Increased activity of the antioxidant enzymes SOD after melatonin administration decreased MDA levels (p<0.05). In summary, melatonin administration benefited the ovarian response and embryo quality in superstimulated Thai-Holstein crossbreed affected by heat stress. 
     Keyword dairy cows, Follicular development, Oxidative Stress, ROS, Superstimulation, THI (Temperature Humidity Index) 
Author
637030011-4 Miss RUTHAIPORN RATCHAMAK [Main Author]
Agriculture Doctoral Degree

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Level of Publication นานาชาติ 
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