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Publication
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| Title of Article |
Micropropagation of pokeweed (Phytolacca americana L.) and comparison of phenolic, flavonoid content, and
antioxidant activity between pokeweed callus and other parts |
| Date of Acceptance |
16 January 2022 |
| Journal |
| Title of Journal |
PeerJ |
| Standard |
SCOPUS |
| Institute of Journal |
PeerJ Inc. |
| ISBN/ISSN |
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| Volume |
10 |
| Issue |
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| Month |
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| Year of Publication |
2022 |
| Page |
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| Abstract |
Background. Pokeweed (Phytolacca americana L.) is regarded as an invasive plant in
many parts of the world but possesses therapeutic characteristics used for antitumor and
rheumatism treatment. This study investigated the effects of auxins and four explants
on pokeweed callus induction. The effects of cytokinins and combinations between
cytokinins and NAA on shoot and root induction were also studied. TPC, TFC and
antioxidant activity of calli were screened and compared with other pokeweed plant
parts.
Methods. Four explants were used to induce callus using 2,4-D and IBA at 1, 2, 3 and
4 mg/l for each auxin. Direct shoot organogenesis from nodal explants was investigated
using BAP, kinetin and TDZ (1, 2 and 4 mg/l for each cytokinin). Combined effects
between cytokinins and NAA at 0.1, 0.2 and 0.3 mg/l were further simultaneously
estimated with root induction. Calli derived from the leaves were compared with other
plant parts for TPC, TFC and antioxidant activity using the Folin-Ciocalteu, AlCl3
colorimetric assay and DPPH assays, respectively.
Results. Results showed that MS medium containing 2 mg/l 2,4-D induced callus
formation on leaf explants that provided highest fresh and dry weights. Three types of
synthetic cytokinins as kinetin, TDZ and BAP were used for direct shoot organogenesis
from pokeweed nodes. MS medium containing 2 mg/l kinetin was effective in
stimulating normal shoots, with the largest number of shoots and leaves and the
longest shoots. The combination between cytokinins and NAA showed no positive
effect on shoot and root induction from pokeweed nodal explants. For TPC and TFC
determination, pokeweed seeds and leaves possessed the highest phenolic and flavonoid
contents, respectively. Highest phenolic content of pokeweed seeds led to lowest IC50
by DPPH assay. Phenolic content was higher than flavonoid content.
Conclusion. Results suggested promising conditions for callus induction. Leaf explants
cultured on MS medium with 2 mg/l 2,4-D and nodal explants cultured on MS
medium with 2 mg/l kinetin provided the largest number of normal shoots and leaves.
NAA did not show positive effects on shoot and root induction when combined with
cytokinins. Chemical constituent screening indicated that seeds and leaves provided highest TPC and TFC, respectively, while pokeweed calli contained higher phenolic
than flavonoid content. This is the first report describing chemical constituent screening
and antioxidant activity of calli and other parts of the pokeweed plant. Results provided
significant information to further enhance bioactive compound contents of pokeweed
calli using elicitation methods. |
| Keyword |
Antioxidant, Callus, Chemical constituents, Micropropagation, Pokeweed |
| Author |
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| Reviewing Status |
มีผู้ประเมินอิสระ |
| Status |
ตีพิมพ์แล้ว |
| Level of Publication |
นานาชาติ |
| citation |
true |
| Part of thesis |
true |
| Attach file |
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| Citation |
0
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Journal Publication
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