| Research Title |
Expression and localization of diacylglycerol kinase iota (DGK-ɩ) in the rat model of acute kidney injury induced by gentamicin |
| Date of Distribution |
25 February 2022 |
| Conference |
| Title of the Conference |
การประชุมวิชาการระดับชาติพิบูลสงครามวิจัย ครั้งที่ 7 ประจำปี พ.ศ. 2565 “การวิจัยและนวัตกรรม เพื่อความยั่งยืนภายใต้ชีวิตวิถีใหม่” |
| Organiser |
สถาบันวิจัยและพัฒนา มหาวิทยาลัยราชภัฏพิบูลสงคราม |
| Conference Place |
มหาวิทยาลัยราชภัฏพิบูลสงคราม (ONLINE CONFERENCE) |
| Province/State |
พิษณุโลก |
| Conference Date |
25 February 2022 |
| To |
25 February 2022 |
| Proceeding Paper |
| Volume |
7 |
| Issue |
1 |
| Page |
592-602 |
| Editors/edition/publisher |
|
| Abstract |
The present study aimed to examine the expression and localization of the diacylglycerol kinase iota (DGK-ɩ) in rat kidneys with the normal condition and gentamicin-induced acute kidney injury (AKI) model using immunohistochemistry and immunoblotting techniques. The results demonstrated the localization of DGK-ɩ in the normal rat proximal renal tubules, particularly on the apical cellular areas occupied with brush borders. It was gradually potent at the early stages of gentamicin-induced AKI for one day and three days, respectively (p < 0.001). However, the immunoreactivity of DGK-ɩ in gentamicin-induced AKI at a later stage as for five days was the significantly weaker intensity (p < 0.001). Moreover, an immunoblotting comparison between the expression of DGK-ɩ and the apoptotic marker cleaved caspase-3 during the early stages of renal tubular injury showed significantly increased both DGK-ɩ and cleaved caspase-3 expressions. Conversely, during the later stage of renal tubular injury, gentamicin-induced AKI for five days, DGK-ɩ expression was significantly decreased while cleaved caspase-3 expression was significantly increased (p < 0.001). The present finding suggests that DGK-ɩ may be involved in proximal tubular cell reabsorption and is involved in the early pathogenesis of AKI. Therefore, DGK-ɩ may be a new biomarker for AKI. However, further investigations must be elucidated.
keywords : diacylglycerol kinase iota, acute kidney injury, renal proximal tubules, rat |
| Author |
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| Peer Review Status |
มีผู้ประเมินอิสระ |
| Level of Conference |
ชาติ |
| Type of Proceeding |
Full paper |
| Type of Presentation |
Oral |
| Part of thesis |
true |
| ใช้สำหรับสำเร็จการศึกษา |
ไม่เป็น |
| Presentation awarding |
true |
| Award Title |
การนำเสนอผลงานแบบบรรยายอยู่ในระดับดี |
| Type of award |
รางวัลด้านวิชาการ วิชาชีพ |
| Organiser |
มหาวิทยาลัยราชภัฏพิบูลสงคราม |
| Date of awarding |
4 มีนาคม 2565 |
| Attach file |
|
| Citation |
0
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Publication
Journal Publication
ไทย