| Research Title |
Interactions of Burkholderia pseudomallei biofilm phenotype with Acanthamoeba sp. |
| Date of Distribution |
27 December 2021 |
| Conference |
| Title of the Conference |
การจัดประชุมวิชาการระดับชาติ ครั้งที่ 18 ในการจัดสัมมนาและประชุมวิชาการงาน เกษตรกำแพงแสน ประจำปี 2564 |
| Organiser |
มหาวิทยาลัยเกษตรศาสตร์ วิทยาเขตกำแพงแสน และกองบริหารการศึกษา |
| Conference Place |
อาคารศูนย์เรียนรวม มหาวิทยาลัยเกษตรศาสตร์ วิทยาเขตกำแพงแสน |
| Province/State |
นครปฐม |
| Conference Date |
8 December 2021 |
| To |
9 December 2021 |
| Proceeding Paper |
| Volume |
18 |
| Issue |
1 |
| Page |
2063-2071 |
| Editors/edition/publisher |
|
| Abstract |
Burkholderia pseudomallei is an etiological agent of melioidosis. Biofilm formation is correlated
with chronic infections, antibiotic resistance and protect bacteria from host immune defense.The aim of
this study was to investigate the responsibility of B. pseudomallei biofilm phenotype against amoeba
grazing during co-cultivation with Acanthamoeba sp. that isolated from B. pseudomallei soil positive
area. B. pseudomallei H777 were co-cultured with Acanthamoeba sp. at a ratio of 100:1 in Page’s
modified-Neff’s amoeba saline (PAS) medium, incubated at 30°C for 3 h. Viability of internalized bacteria
was determined using a drop plate technique. Moreover, B. pseudomallei biofilms were cocultured with
~103
amoeba cells/ml for another 24 h. The biofilm formation was monitored using confocal laser
scanning microscopy (CLSM). The amoeba numbers were counted. The results revealed the survival of
internalized B. pseudomallei were significantly (p < 0.001) decrease from 3.61 ± 0.24 Log10CFU/ml at 0
h to 2.10 ± 0.09 Log10CFU/ml at 3 h. Furthermore, biofilm biomass of B. pseudomallei (1.39 ± 0.03
µm
3
/µm2
) was significantly (p < 0.001) reduced compared to the untreated control (1.05 ± 0.04
µm
3
/µm2
). In addition, amoeba cells were increased to 1.95 ± 0.14 x103
cells/ml. These data suggest
the ability of Acanthamoeba sp. to graze B. pseudomallei biofilm phenotypes as food sources and
facilitate amoeba multiplication. |
| Author |
|
| Peer Review Status |
มีผู้ประเมินอิสระ |
| Level of Conference |
ชาติ |
| Type of Proceeding |
Full paper |
| Type of Presentation |
Oral |
| Part of thesis |
true |
| Presentation awarding |
false |
| Attach file |
|
| Citation |
0
|
|
|
Publication
Journal Publication
ไทย