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ข้อมูลการเผยแพร่ผลงาน
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| ชื่อบทความ |
Screening of Cyanide-Utilizing Bacteria from Rumen and
In Vitro Evaluation of Fresh Cassava Root Utilization with
Pellet Containing High Sulfur Diet |
| วัน/เดือน/ปี ที่ได้ตอบรับ |
11 มกราคม 2564 |
| วารสาร |
| ชื่อวารสาร |
Veterinary Sciences |
| มาตรฐานของวารสาร |
ISI |
| หน่วยงานเจ้าของวารสาร |
MDPI AG - Multidisciplinary Digital Publishing Institute |
| ISBN/ISSN |
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| ปีที่ |
2021 |
| ฉบับที่ |
8:10 |
| เดือน |
January |
| ปี พ.ศ. ที่พิมพ์ |
2564 |
| หน้า |
1-14 |
| บทคัดย่อ |
Two experiments were undertaken to screen for ruminal cyanide-utilizing bacteria
(Experiment 1), and to evaluate the influence of fresh cassava root (FCR) and pellets containing
high sulfur (PELFUR) on cyanide content, gas production parameters, in vitro degradability, and ruminal fermentation (Experiment 2). Experiment 1 was conducted in a completely randomized
design (CRD) for the screening of cyanide-utilizing bacteria and the dietary treatments consisted of
cyanide at 0, 150, 300, and 450 ppm. In Experiment 2, a 5 × 3 factorial arrangement in a completely
randomized design was used for the in vitro study. Factor A was the level of FCR at 0, 260, 350,
440, and 530 g/kg of dry matter (DM) substrate, and factor B was the level of PELFUR at 0, 15,
and 30 g/kg DM substrate. In Experiment 1, adding different doses of cyanide significantly affected
cyanide-utilizing rumen bacterial growth (p < 0.05). Increasing the concentration of cyanide from 0 to
150 and 150 to 300 ppm resulted in increases in cyanide-utilizing rumen bacteria of 38.2% and 15.0%,
respectively. In Experiment 2, no interaction effects were found between FCR and PELFUR doses
on gas production parameters (p > 0.05). Increasing the FCR level to more than 260 g/kg of DM
substrate could increase cumulative gas production (p < 0.05). Increasing doses of PELFUR from 15
to 30 g/kg increased the cumulative gas production when compared with that of 0 g PELFUR/kg of
DM substrate (p < 0.05). The cyanide concentration in rumen fluid decreased with PELFUR (p < 0.05)
supplementation. Degradability of in vitro DM and organic matter following incubation increased
at 12 and 24 h due to PELFUR supplementation with FCR and increased additionally with 15 g
PELFUR/kg of DM substrate (p < 0.05) in 440 g FCR/kg of DM substrate. Proportions of the total
volatile fatty acids, acetic acid (C2), propionic acid (C3), and butyric acid among supplementations
with FCR (p < 0.05) were significantly different. In conclusion, the present results represent the first
finding of bacteria in the rumen that are capable of utilizing cyanide, and suggests that cyanide might
function as a nitrogen source for bacterial cell synthesis. The inclusion of FCR of 530 g/kg with 30 g
PELFUR/kg of DM substrate could increase the cumulative gas production, the bacterial population,
the in vitro degradability, the proportion of C3, and the rate of the disappearance of cyanide. |
| คำสำคัญ |
thiocyanate, cyanide-utilizing bacteria, rumen fermentation, cyanide, degradability |
| ผู้เขียน |
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| การประเมินบทความ |
มีผู้ประเมินอิสระ |
| สถานภาพการเผยแพร่ |
ตีพิมพ์แล้ว |
| วารสารมีการเผยแพร่ในระดับ |
นานาชาติ |
| citation |
มี |
| เป็นส่วนหนึ่งของวิทยานิพนธ์ |
เป็น |
| แนบไฟล์ |
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| Citation |
0
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