| Research Title |
THE EFFECT OF VICENIN-2 ON LPS-INDUCED AUTOPHAGY IN HUMAN GINGIVAL FIBROBLASTS |
| Date of Distribution |
29 April 2022 |
| Conference |
| Title of the Conference |
Thai Society for Biotechnology International Conference Online |
| Organiser |
Thai Society for Biotechnology (TSB) |
| Conference Place |
Bangkok, Thailand |
| Province/State |
Bangkok |
| Conference Date |
29 April 2022 |
| To |
29 April 2022 |
| Proceeding Paper |
| Volume |
1 |
| Issue |
1 |
| Page |
98-103 |
| Editors/edition/publisher |
|
| Abstract |
The objectives of this study were to investigate whether lipopolysaccharide of P. gingivalis (LPS-Pg) could induce autophagy in human gingival fibroblasts (HGFs) and to analyze whether Vicenin-2 (VCN-2), a flavonoid glycoside, exhibited any effect on autophagy induced by LPS-Pg in HGFs. The PrestoBlue assay demonstrated that different concentrations of LPS-Pg (1.5-100 µg/mL) and VCN-2 (1.5-400 µM) did not significantly affect the cell viability of HGFs. Among all concentrations tested, 5 µg/mL of LPS-Pg showed the highest induction of autophagy in HGFs, confirmed by an increase in the protein expression levels of LC3B-II/LC3B-I ratio and a reduction of p62 protein levels. The immunofluorescent study demonstrated that VCN-2 at concentrations of 12.5 and 25 μM can increase the levels of p62 protein in LPS-Pg-treated HGFs. These results preliminarily provide evidence of VCN-2’s ability to inhibit the induction of autophagy induced by LPS-Pg, suggesting the potential use of VCN-2 to be a novel alternative modality for periodontal disease treatment. |
| Author |
|
| Peer Review Status |
มีผู้ประเมินอิสระ |
| Level of Conference |
นานาชาติ |
| Type of Proceeding |
Full paper |
| Type of Presentation |
Oral |
| Part of thesis |
true |
| Presentation awarding |
false |
| Attach file |
|
| Citation |
11
|
|
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Publication
Journal Publication
ไทย