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Publication
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| Research Title |
Estrogenic activity of isoflavones derived from Derris scandens using MCF-7 cell |
| Date of Distribution |
31 August 2022 |
| Conference |
| Title of the Conference |
70th International Congress and Annual Meeting of the Society for Medicinal Plant and Natural Product Research (GA2022) |
| Organiser |
Society for Medicinal Plant and Natural Product Research (GA) |
| Conference Place |
Thessaloniki Concert Hall |
| Province/State |
Thessaloniki, Greece |
| Conference Date |
5 October 2022 |
| To |
5 October 2022 |
| Proceeding Paper |
| Volume |
- |
| Issue |
- |
| Page |
- |
| Editors/edition/publisher |
- |
| Abstract |
Derris scandens (Hog Creeper Vine), commonly known as "Thao Wan Priang" in Thai, is a woody climbing vine in the Fabaceae family. This herb possesses analgesic and anti-inflammatory effects. Isoflavonoids and their prenylated derivatives are the major compounds found in D. scandens. The chemical markers for this plant are genistein-7-O-[-rhamnopyranosyl-(1 to 6)-glucopyranoside] (GTG) and genistein, according to the Thai Herbal Pharmacopoeia (THP). Isoflavonoids are phytoestrogens present in fabaceous plants that can act as estrogen replacements. Therefore, this study aimed to evaluate the estrogenic activity of GTG and lupalbigenin derived from D. scandens. In the present study, we used an E-screening proliferation assay in the MCF-7 cell line and RT-PCR to investigate gene expression. The results showed that 1 µM GTG induced 71.15% proliferation compared to 0.1 nM 17β-estradiol (set up to 100% relative proliferative effect) while 1 µM lupalbigenin reduced proliferation to 18.72%. Furthermore, when 1 µM lupalbigenin was co-treated with 0.1 nM 17β-estradiol, cell proliferation was altered to 80.38%. The results suggest that lupalbigenin has anti-estrogen effect in vitro. The effect of GTG and lupalbigenin on gene expression levels were analyzed. GTG increased estrogen receptor gene expression (2.5-fold), while androgen receptor and TMPRSS2 gene expression were suppressed. Lupalbigenin significantly inhibited the androgen receptor on transcriptional level. Both compounds might contribute to the estrogenic effect of D. scandens extracts. However, further studies should be performed to confirm estrogenic activity of plant extracts. |
| Author |
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| Peer Review Status |
มีผู้ประเมินอิสระ |
| Level of Conference |
นานาชาติ |
| Type of Proceeding |
Abstract |
| Type of Presentation |
Poster |
| Part of thesis |
true |
| Presentation awarding |
false |
| Attach file |
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| Citation |
2
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Journal Publication
ไทย