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Publication
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| Title of Article |
Direct chromosomal preparation protocol from old world tarantulas (Araneae, Theraphosidae) |
| Date of Acceptance |
28 December 2022 |
| Journal |
| Title of Journal |
Science Technology and Engineering Journal (STEJ) |
| Standard |
TCI |
| Institute of Journal |
Division of Research Facilitation and Dissemination, Mahasarakham University, Kham Riang, Kantharawichai, Maha Sarakham, Thailand 44150 |
| ISBN/ISSN |
2697-6110 |
| Volume |
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| Issue |
8(2) |
| Month |
ธันวาคม |
| Year of Publication |
2022 |
| Page |
111–118 |
| Abstract |
The Theraphosidae (Thorell, 1869) is the most prevalent family in the suborder Mygalomorpae, but little is known about their cytogenetics, nor have the chromosomes of old-world tarantulas been studied. This study aims to develop an efficient protocol for chromosomal tarantula preparation in which chromosomes are extracted directly from the internal organs of spiders following in vivo colchicine treatment. Chilobrachys (Karsch, 1892) and Cyriopagopus (Simon, 1888), which belong to different subfamilies, were chosen as validation models. The results indicate that the protocol for preparing chromosomes from both subfamilies is effective, suggesting that Southeast Asian tarantulas are probable to have bi-arms chromosomes and a high proportion of diploid number (2n). Interestingly, diploid chromosomes contain chromosomal gaps on many chromosomes, which will be investigated in the future to facilitate a greater understanding. |
| Keyword |
Chromosome Chilobrachys Cyriopagopus Selenocosminae Ornithoctoninae |
| Author |
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| Reviewing Status |
มีผู้ประเมินอิสระ |
| Status |
ตีพิมพ์แล้ว |
| Level of Publication |
ชาติ |
| citation |
false |
| Part of thesis |
true |
| Attach file |
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| Citation |
0
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Journal Publication
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