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Publication
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| Title of Article |
Electrochemical biosensor based on cellulose nanofibers/graphene oxide and acetylcholinesterase for the detection of chlorpyrifos pesticide in water and fruit juice |
| Date of Acceptance |
2 March 2023 |
| Journal |
| Title of Journal |
RSC advances |
| Standard |
SCOPUS |
| Institute of Journal |
Royal Society of Chemistry (RSC) |
| ISBN/ISSN |
- |
| Volume |
2023 |
| Issue |
- |
| Month |
March |
| Year of Publication |
2023 |
| Page |
RSC Adv., 2023, 13, 9603–9614 |
| Abstract |
In this work, cellulose nanofibers and graphene oxide are used to fabricate a simple and reliable
electrochemical biosensor, based on acetylcholinesterase (AChE) for the detection of highly dangerous
organophosphates (OPs), utilizing chlorpyrifos as a representative sample. AChE is an enzyme that is
essential for neurotransmission and catalyzes the conversion of acetylcholine (ATCh) into thiocholine
and acetic acid. The pesticide used in this work, chlorpyrifos, inhibits the catalytic activity of AChE on
ATCh, and this inhibition can be measured using square wave voltammetry (SWV). Utilizing a process of
surface modification, layers of cellulose nanofibers, graphene oxide, a chitosan-graphene oxide
composite, and acetylcholinesterase (AChE/CS-GO/GO/CNFs) were immobilized on a screen-printed
carbon electrode (SPCE). The modified SPCE working electrode was characterized using scanning
electron microscopy and graphene oxide trapped in the cellulose nanofibers was found to increase the
sensitivity of the biosensor. The modified biosensor demonstrated good performance for detection of
chlorpyrifos over a linear range of 25–1000 nM under optimum conditions, and the limits of detection
and quantification were 2.2 nM and 73 nM, respectively. Our sophisticated technique might offer a more
precise, straightforward, quick, and environmentally friendly way to assess chlorpyrifos contamination in
water and juice samples. |
| Keyword |
cellulose nanofibers, acetylcholinesterase, acetylthiocholine, chlorpyrifos, biosensor, square wave voltammetry, screen-printed carbon electrode |
| Author |
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| Reviewing Status |
มีผู้ประเมินอิสระ |
| Status |
ตีพิมพ์แล้ว |
| Level of Publication |
นานาชาติ |
| citation |
true |
| Part of thesis |
true |
| Attach file |
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| Citation |
0
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Journal Publication
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