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Publication
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| Title of Article |
Serum α2,6-sialylated glycoform of serotransferrin as a glycobiomarker for diagnosis and prediction of clinical severity in cholangiocarcinoma |
| Date of Acceptance |
11 September 2022 |
| Journal |
| Title of Journal |
Clinica Chimica Acta |
| Standard |
SCOPUS |
| Institute of Journal |
ELSEVIER |
| ISBN/ISSN |
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| Volume |
2022 |
| Issue |
536 |
| Month |
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| Year of Publication |
2022 |
| Page |
142-154 |
| Abstract |
Background: Glycoprotein sialylation changes are associated with severe development of various cancers. We
previously discovered the sialylation of serotransferrin (TF) in cholangiocarcinoma (CCA) using glycoproteomics
approach. However, a simple and reliable method for validating sialylation of a specific glycobiomarker is urgently needed.
Methods: We identified the altered glycosylation in CCA tissues by glycoproteomics approach using mass spectrometry. An enzyme-linked lectin assay (ELLA) was developed for determining the serum levels of sialylated TF
in CCA, hepatocellular carcinoma (HCC) and healthy controls in training and validation cohorts.
Results: The nine highly sialylated glycoforms of TF were markedly abundant in CCA tumor tissues than in
control. Serum SNA-TF and MAL1-TF were significantly higher in CCA patients. Under receiver operating
characteristic curve, serum SNA-TF concentrations significantly differentiated CCA from healthy control. Higher
SNA-TF were significantly correlated with severe tumor stages and lymph node metastasis. The combined SNATF, MAL1-TF, and CA19-9 as a novel glycobiomarkers panel demonstrated the highest specificity (96.2%) for
distinguishing CCA from HCC patients. In CCA patients with low CA19-9 levels, SNA-TF in combination with
CA19-9 achieved in 97% diagnostic accuracy.
Conclusions: Sialylated serotransferrin glycoforms could be used as a novel glycobiomarker for diagnosis and
prediction of clinical severity in CCA patients. |
| Keyword |
Cholangiocarcinoma |
| Author |
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| Reviewing Status |
มีผู้ประเมินอิสระ |
| Status |
ตีพิมพ์แล้ว |
| Level of Publication |
นานาชาติ |
| citation |
true |
| Part of thesis |
true |
| Attach file |
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| Citation |
0
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Journal Publication
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