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Publication
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| Title of Article |
Occurrence of ciprofloxacin resistance, plasmid-mediated quinolone resistance genes and virulence factors in Salmonella enterica serovar Enteritidis isolated from broiler farms in the central and northeastern parts of Thailand |
| Date of Acceptance |
12 April 2023 |
| Journal |
| Title of Journal |
Veterinary Integrative Science |
| Standard |
SCOPUS |
| Institute of Journal |
The Faculty of Veterinary Medicine, Chiang Mai University, Thailand |
| ISBN/ISSN |
ISSN as 2629-9968 |
| Volume |
21 |
| Issue |
2 |
| Month |
May-August |
| Year of Publication |
2023 |
| Page |
xx-xx |
| Abstract |
Salmonella Enteritidis is one of the most common serovars associated with food-prisoning and gastroenteritis in humans. Fluoroquinolone resistance in non-typhoidal Salmonella including S. Enteritidis has been increasing globally and is considered as an urgent threat to public health. In this study, we aimed to investigate the occurrence of ciprofloxacin resistance and plasmid-mediated quinolone resistance (PMQR) genes, and to examine the virulence gene profiles of 69 S. Enteritidis isolates. The isolates were obtained from 47 boot swab and 22 intestinal content samples, collected from 69 Good Agricultural Practice (GAP)- certified broiler farms located in the central and northeastern regions of Thailand. One isolate was randomly selected from each farm for analysis. Ciprofloxacin susceptibility of these isolates was determined using microbroth dilution method. PCR was used to detect 5 common PMQR genes (qnrA, qnrB, qnrS, aac(6')-Ib-cr and qepA) and 12 important virulence genes (agfA, invA, spaN, prgH, sitC, ssaQ, mgtC, sopB, sifA, tolC, cdtB and spvC). All S. Enteritidis showed reduced susceptibility to ciprofloxacin, with the MIC values of 0.125-0.50 µg/mL. However, these isolates did not carry PMQR genes investigated. All 69 S. Enteritidis isolates exhibited an identical virulence profile, characterized by the presence of 11 virulence genes, except for cdtB. The presence of virulence genes identified in invasive salmonellosis among S. Enteritidis isolates with reduced susceptibility to ciprofloxacin could pose public health concerns. Our findings underline the need for constant monitoring of ciprofloxacin-resistant S. Enteritidis in the poultry production chain to reduce public health risk. |
| Keyword |
Antibiotic resistance, Salmonella Enteritidis, broiler farms, virulence genes, Plasmid-Mediated Quinolone Resistance Genes, Thailand |
| Author |
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| Reviewing Status |
มีผู้ประเมินอิสระ |
| Status |
ได้รับการตอบรับให้ตีพิมพ์ |
| Level of Publication |
นานาชาติ |
| citation |
false |
| Part of thesis |
true |
| Attach file |
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| Citation |
0
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Journal Publication
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