| Research Title |
Cyclodextrin and cellulase induced isoflavonoids production in Pueraria candollei cell suspension culture |
| Date of Distribution |
21 May 2023 |
| Conference |
| Title of the Conference |
Phytochemical Society of Europe (PSE) Meeting 2023: “Natural compounds as regulators of molecular mechanisms in health and disease” |
| Organiser |
Recherches Scientifiques Luxembourg asbl, The "Natural Products Research Institute" |
| Conference Place |
College of Pharmacy of Seoul National University |
| Province/State |
South Korea |
| Conference Date |
19 May 2023 |
| To |
21 May 2023 |
| Proceeding Paper |
| Volume |
- |
| Issue |
- |
| Page |
- |
| Editors/edition/publisher |
|
| Abstract |
Introductions: Pueraria candollei (Leguminosae) has been used in Thai traditional medicines and treatment of menopausal symptoms. The major phytoestrogen compounds isolated from this intact plant and callus are isoflavonoids [1]. Elicitors are used for enhancing secondary metabolites in plant tissue culture. Cellulase is an enzyme from bacteria and fungi which has degraded function of plant cell wall. Isoflavonoid glycosides are transformed to aglycone that is the benefit of cellulase on isoflavonoid extraction [2]. Another elicitor is 2-hydroxypropyl-β-cyclodextrin (HBCD) that uses for increasing the solubilization and accumulation of secondary metabolites in plant tissue cultures [3]. This work aims to study effect of cellulase and HBCD on isoflavonoids production. Materials and methods: Three grams of P. candollei cell suspension was cultured in 3% sucrose MS liquid media supplied with 0.1 mg/L TDZ, 1 mg/L NAA and 0.5 mg/L BA (T0.1N1B0.5 MS media) for 3 weeks [1]. Then, the cell suspension was transferred to the same media plus with 33.6 mU/mL cellulase [2] or 50 µM HBCD [3]. Medium without elicitor was used as control. Treated cell suspension was harvested at day 3, 6, 9, 12 and 20. All samples were determined isoflavonoids levels by HPLC-UV. Field-emission scanning electron microscope (FE-SEM) was used for investigation of treated cell appearances at day 20. Results and discussion: At day 20 of cell treated with HBCD showed daidzin, daidzein, genistin and genistein were 1571.85, 224.38, 287.94 and 17.40 µg/g dry weight, respectively. Maximum total isoflavonoids production of cellulase condition was limited at day 12. The elicited cell suspension at day 20 of HBCD condition was gained highest isoflavonoids production and it also released into media. FE-SEM showed HBCD effect to porous on surface of the P. candollei cells. Our study indicated HBCD is an optimal elicitor for increasing isoflavonoids production in cell and can be released into media for harvesting. Mechanism of HBCD on gene expression in isoflavonoid biosynthesis pathway of P. candollei cell culture should be investigated for further study. |
| Author |
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| Peer Review Status |
มีผู้ประเมินอิสระ |
| Level of Conference |
นานาชาติ |
| Type of Proceeding |
Abstract |
| Type of Presentation |
Oral |
| Part of thesis |
true |
| Presentation awarding |
false |
| Attach file |
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| Citation |
0
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Publication
Journal Publication
ไทย