| Abstract |
Helminth defense molecules (HDMs) are secreted by parasitic flatworms. HDMs showed immunomodulatory activity by suppressing LPS-induced inflammatory response. Modified HDM from the liver fluke Fasciola hepatica showed anti-bacterial and anti-cancer activities. This study aims to evaluate the activity of OvHDM of Opisthorchis viverrini. The OvHDM, modified OvHDM, modified FhHDM-1 and LL37-fragment were tested against bacteria and cancer cells. The broth microdilution was used to measure anti-bacterial activity against Escherichia coli and Staphylococcus epidermidis. We found that OvHDM and modified OvHDM exhibited no anti-microbial effect. In contrast, modified FhHDM-1 showed effect to against E. coli and S. epidermidis at 12.5 and 3.12 µM, respectively. Moreover, it showed bactericidal to E. coli and S. epidermidis at 50 and 25 µM, respectively. Likewise, the LL-37 fragment inhibited E. coli and S. epidermidis at 25 and 50 µM respectively. Moreover, the LL-37 fragment showed a bactericidal effect against E. coli at 100 µM. however, but not on S. epidermidis. The MTT assay was used to observe cytotoxicity on KKU100 (CCA) and H69 (normal cells). The result showed that OvHDM and modified OvHDM did not affect both cells. However, modified FhHDM-1 and LL-37 fragments displayed a killing effect on KKU100 but not on H69 cells with EC50 at 11 and 33.8 µM, respectively. In conclusion, HDM and modified HDM from O. viverrini showed no anti-microbial and anti-cancer activities. Further studies are required to develop the anti-cancer and anti-bacterial peptides from the liver fluke O. viverrini HDM as it has been successful for F. hepatica. |
Publication
Journal Publication
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