| Research Title |
Development of Chicken IgY-based Indirect ELISA for detect Opisthorchis viverrini antigen in feces |
| Date of Distribution |
25 May 2023 |
| Conference |
| Title of the Conference |
14th Science Research Conference |
| Organiser |
School of Science, University of Phayao |
| Conference Place |
University of Phayao |
| Province/State |
Phayao Province |
| Conference Date |
25 May 2023 |
| To |
26 May 2023 |
| Proceeding Paper |
| Volume |
2566 |
| Issue |
- |
| Page |
online |
| Editors/edition/publisher |
|
| Abstract |
The human liver fluke is a significant public health concern affecting over 600 million individuals worldwide. Efforts to control liver fluke infection in the Greater Mekong Subregion are ongoing, and include public health education campaigns, improved sanitation and hygiene, and the use of anthelmintic drugs to treat infected individuals. However, more needs to be done to address this significant public health concern and reduce the burden of liver fluke infection including new diagnostic method development. Despite efforts to develop more sensitive and specific detection methods, diagnosis is still largely based on traditional stool examination. In this study, we developed an indirect ELISA using anti- O. viverrini cathepsin F (rOv-CF) IgY antibody to detect O. viverrini antigen in human stool. We used the TCA stool processing method to prepare the OV-somatic antigen spiked stool to analyze. As a result, all standard curves of this indirect ELISA are parallel which indicate this method is reliable and consistent for measuring OV-somatic antigen concentration in human stool (R2=0.99, P=0.987) with limit of detection of 2.2 μg/ml (OV-somatic antigen). The study can be the base line for further research of OV antigen detection in human stool, and use for screening O. viverrini infection in endemic area in the further. |
| Author |
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| Peer Review Status |
มีผู้ประเมินอิสระ |
| Level of Conference |
ชาติ |
| Type of Proceeding |
Full paper |
| Type of Presentation |
Poster |
| Part of thesis |
true |
| ใช้สำหรับสำเร็จการศึกษา |
ไม่เป็น |
| Presentation awarding |
true |
| Award Title |
Excellence Poster Presentation |
| Type of award |
รางวัลด้านกีฬาและสุขภาพ |
| Organiser |
School of Science, University of Phayao |
| Date of awarding |
25 พฤษภาคม 2566 |
| Attach file |
|
| Citation |
0
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Publication
Journal Publication
ไทย