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Publication
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| Research Title |
Population genetic of cat- and human-specific
Opisthorchis viverrini in cyprinid fish |
| Date of Distribution |
20 July 2023 |
| Conference |
| Title of the Conference |
The 24th Khon Kaen Veterinary Annual International Conference (KVAC) 2023 “Global Animal Health, Nutrition technology and Innovation” |
| Organiser |
Faculty of Veterinary Medicine, KKU |
| Conference Place |
AVANI Khon Kaen Hotel & Convention Centre Khon Kaen, Thailand |
| Province/State |
Khon Kaen |
| Conference Date |
20 July 2023 |
| To |
21 July 2023 |
| Proceeding Paper |
| Volume |
24 |
| Issue |
1 |
| Page |
62-71 |
| Editors/edition/publisher |
Peerapol Sukon, Prawit Butudom, Ranee Singh |
| Abstract |
Objective: To study the population genetic of cat- and human-specific Opisthorchis viverrini (O. viverrini)
in cyprinid fish in Khammouane, Lao PDR and Udon Thani, Thailand.
Materials and Methods Cyprinid sp. fish were collected from Khammouane, Lao PDR and Udon Thani,
Thailand from July 2022 to February 2023. Fish were digested using homogenization and an acid-pepsin
digestion procedure. Finally, a stereomicroscope was used to isolate and identify the metacercariae in the
silt. O. viverrini-like metacercariae were isolated individually and added 1X PBS in 1.5 ml microcentrifuge
tube. Individual O. viverrini-like metacercaria was extracted and positive O. viverrini metacercariae were
sent for sequencing. Cytochrome c oxidase subunit 1 (cox1) was used to investigate genetic variation in the
present study.
Results: The sequencing results; The phylogenetic tree analysis of 91 and 53 individual O. viverrini metacercariae from Khammouane, Lao PDR and Udon Thani, Thailand, respectively revealed that all O. viverrini
metacercariae in cyprinid fish belonged to human-specific O. viverrini. Cat-specific O. viverrini could not
be found in cyprinid fish from Udon Thani and Khammouane. We got 99.25 to 100% identity with humanspecific or widespread cluster O. viverrini from BLASTN analysis. The results were also supported by the
median-joining haplotype network analysis.
Conclusion Our results revealed that all O. viverrini metacercariae from cyprinid fish in Khammouane, Lao
PDR and Udon Thani, Thailand, belonged to human-specific O. viverrini. None of our samples belonged to
cat-specific O. viverrini. We assumed that the cox1 nucleotide sequence from cat-specific O. viverrini metacercariae which is identical to cat-specific O. viverrini eggs cannot be found in the environment, due to small
amounts of sample size or possibly harbored in the different secondary intermediate hosts. In our opinion,
cyprinid fish seems to be accidental host of cat-specific O. viverrini metacercariae. Further investigation is
needed to answer the question; what and where is the main secondary intermediate host of cat-specific O.
viverrini?. |
| Author |
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| Peer Review Status |
มีผู้ประเมินอิสระ |
| Level of Conference |
นานาชาติ |
| Type of Proceeding |
Full paper |
| Type of Presentation |
Oral |
| Part of thesis |
true |
| Presentation awarding |
true |
| Award Title |
Best Oral Presentation Award_1st Place |
| Type of award |
รางวัลด้านกีฬาและสุขภาพ |
| Organiser |
Faculty of Veterinary Medicine, KKU |
| Date of awarding |
21 กรกฎาคม 2566 |
| Attach file |
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| Citation |
0
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Journal Publication
ไทย