| Abstract |
This work demonstrates the ability of the Ion-Sensitive Field-Effect Transistor (ISFET)-
based immunosensor to detect antibodies against the human leukocyte antigen (HLA) and the
major histocompatibility complex class-I-related chain A (MICA). The sensing membrane of the
ISFET devices was modified and functionalized using an APTES-GA strategy. Surface properties,
including wettability, surface thickness, and surface topology, were assessed in each module of the
modification process. The optimal concentrations of HLA and MICA proteins for the immobilization
were 10 and 50 g/mL. The dose-response curve showed a detection range of 1.98–40 g/mL for
anti-HLA and 5.17–40 g/mL for anti-MICA. The analytical precision (%CV) was found to be 10.69%
and 8.92% for anti-HLA and -MICA, respectively. Moreover, the electrical signal obtained from the
irrelevant antibody was considerably different from that of the specific antibodies, indicating the
specific binding of the relevant antibodies without noise interference. The sensitivity and specificity
in the experimental setting were established for both antibodies (anti-HLA: sensitivity = 80.00%,
specificity = 86.36%; anti-MICA: sensitivity = 86.67%, specificity = 88.89%). Our data reveal the
potential of applying the ISFET-based immunosensor to the detection of relevant anti-HLA and
-MICA antibodies, especially in the field of kidney transplantation. |
Publication
Journal Publication
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