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Publication
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| Research Title |
Peptide Derived from Progranulin of The Carcinogenic
Liver Fluke, Opisthorchis viverrini Stimulates Cell
Hyperproliferation and Proinflammatory Cytokine
Production |
| Date of Distribution |
31 August 2023 |
| Conference |
| Title of the Conference |
37th Annual Conference MEDICINE KKU 2023 |
| Organiser |
คณะแพทยศาสตร์ มหาวิทยาลัยขอนแก่น |
| Conference Place |
อาคารเรียนรวม ชั้น 3 คณะแพทยศาสตร์ มหาวิทยาลัยขอนแก่น |
| Province/State |
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| Conference Date |
7 September 2023 |
| To |
8 September 2023 |
| Proceeding Paper |
| Volume |
37 |
| Issue |
Supplement |
| Page |
1-15 |
| Editors/edition/publisher |
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| Abstract |
Background and Objective: Progranulin (PGRN) is a secreted glycoprotein growth factor with roles in wound
healing, inflammation, angiogenesis and malignancy. An orthologue of the gene encoding human PGRN was
identified in the carcinogenic liver fluke, Opisthorchis viverrini.
Methods: Sequence structure, general characteristics and possible function of Opisthorchis viverrini PGRN
(Ov-PGRN) were analyzed using bioinformatics. Expression profiles were investigated with quantitative reverse
transcription polymerase chain reaction (RT-PCR), western blot and immunolocalization. A specific peptide of
Ov-PGRN was used to investigate a role for this molecule in pathogenesis.
Results: The structure of the gene coding for Ov-PGRN was 36,463 base pairs in length, and comprised of 13 exons,
12 introns, and a promoter sequence. The Ov-PGRN mRNA is 2,768 base pairs in length and encodes 846 amino
acids with a predicted molecular mass of 91.61 kDa. Ov-PGRN exhibited one half and seven complete granulin
domains. Phylogenetic analysis revealed that Ov-PGRN formed its closest relationship with PGRN of liver flukes
in the Opisthorchiidae. Transcripts of Ov-PGRN were detected in several developmental stages, with highest
expression in the metacercaria, indicating that Ov-PGRN may participate as a growth factor in the early
development of O. viverrini. Western blot analysis revealed the presence of detected Ov-PGRN in both soluble
somatic or excretory/secretory products, and immunolocalization indicated high levels of expression in the tegument
and parenchyma of the adult liver fluke. Co-culture of a human cholangiocyte cell line and a peptide
fragment of Ov-PGRN stimulated proliferation of cholangiocytes and upregulation of expression of the cytokines
interleukins 6 (IL6) and interleukins 8 (IL8).
Conclusion: Ov-PGRN is expressed throughout the life cycle of liver fluke, and likely plays a key role in
development and growth of human cholangiocytes.
Keywords: Opisthorchis viverrini, liver fluke, progranulin, cholangiocyte, cytokines |
| Author |
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| Peer Review Status |
ไม่มีผู้ประเมินอิสระ |
| Level of Conference |
ชาติ |
| Type of Proceeding |
Full paper |
| Type of Presentation |
Oral |
| Part of thesis |
true |
| Presentation awarding |
true |
| Award Title |
รางวัลรองชนะเลิศอันดับที่ 1 |
| Type of award |
รางวัลด้านวิชาการ วิชาชีพ |
| Organiser |
คณะแพทยศาสตร์ มหาวิทยาลัยขอนแก่น |
| Date of awarding |
8 กันยายน 2566 |
| Attach file |
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| Citation |
0
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Journal Publication
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