| Research Title |
Biofilm Formation of Burkholderia pseudomallei H777 Strain in Roswell Park Memorial Institute (RPMI), Dulbecco's Modified Eagle Media (DMEM), and Luria-Bertani (LB) |
| Date of Distribution |
20 September 2023 |
| Conference |
| Title of the Conference |
Multidisciplinary เราเป็นทีมเพื่อประชาชน |
| Organiser |
คณะแพทยศาสตร์ มหาวิทยาลัยขอนแก่น |
| Conference Place |
คณะแพทยศาสตร์ มหาวิทยาลัยขอนแก่น |
| Province/State |
ขอนแก่น |
| Conference Date |
7 September 2023 |
| To |
8 September 2023 |
| Proceeding Paper |
| Volume |
38 |
| Issue |
37 |
| Page |
29-36 |
| Editors/edition/publisher |
ศ.พญ.สุวรรณา อรุณพงค์ไพศาล |
| Abstract |
Background and objective: Burkholderia pseudomallei is the causative agent of melioidosis, a potentially fatal infectious disease worldwide. The disease has a high mortality rate if appropriate treatment is delayed. B. pseudomallei biofilm has been reported as a virulence factor to promote pathogen resistance against host immune response and antibiotics and correlate with relapse melioidosis. Previous research has shown that B. pseudomallei H777, a biofilm producing strain co-cultured with human polymorphonuclear neutrophils (PMNs) in Roswell Park Memorial Institute (RPMI) medium induced neutrophil extracellular traps (NETs) but failed to eradicate the pathogen. The aim of this study was to examine the biofilm formation ability of B. pseudomallei in cell culture medium and optimize the bacterial inoculum for further study to investigate the effects of NETs on B. pseudomallei biofilm formation.
Method: B. pseudomallei was cultured in RPMI or Dulbecco's Modified Eagle media (DMEM) with or without 10% calf bovine serum, in comparison to Luria-Bertani (LB) broth. The inoculum of B. pseudomallei H777 at optical density 600 nm (OD600) of 1and 0.1 were examined to determine the optimal conditions for further study in the co-cultivation with PMNs, considering the potential enhancement through constitutive DNA effects from NETs.
Results: B. pseudomallei H777 cultured in RPMI or DMEM with or without serum, failed to exhibit biofilm formation. In contrast, biofilm formation was observed when cultured in the control LB medium. Additionally, a similar biofilm formation was demonstrated when using B. pseudomallei starter culture at OD600 0.1 and 0.1 in LB medium (p = 0.0799).
Conclusion: LB medium promotes biofilm formation of B. pseudomallei, while RPMI and DMEM media, with or without serum, do not. Cultivating B. pseudomallei starter cultures at OD600 0.1 is suitable for co-cultivation with PMNs to investigate the impact of NETs on biofilm promotion in B. pseudomallei. |
| Author |
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| Peer Review Status |
มีผู้ประเมินอิสระ |
| Level of Conference |
ชาติ |
| Type of Proceeding |
Full paper |
| Type of Presentation |
Oral |
| Part of thesis |
true |
| Presentation awarding |
false |
| Attach file |
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| Citation |
0
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Publication
Journal Publication
ไทย