| Abstract |
Introduction: Monoamine oxidases (MAOs) in humans have two isoforms: MAO-A and MAO-B, which are found in neurons and astroglia. Inhibiting these enzymes can increase
neurotransmitter levels and relieve symptoms of depressive disorder. The Suk Sai-Yad (SSY)
remedy was effective in treating sedative-hypnotic, analeptic, tonic, and panacea conditions
from the forty-fourth traditional medicine recipe recorded in "The Royal Scripture of King
Narai's Medicines". However, there have been no scientific reports of its inhibitory effect on
the MAOs enzyme, which plays an important role in serotonin neurotransmitters. Objective:
This study aimed to investigate the antidepressant-like effect of SSY on the inhibitory effect
on MAOs and on behavioral models of depression, a forced swimming test (FST), and a tail
suspension test (TST). Materials and Methods: In vitro studies using human recombinant
MAO-A and MAO-B enzymes were performed for screening MAOIs in the SSY remedy.
IC50 values were determined for these chimeric enzymes compared with MAO-selective
substrates, clogyrine and deprenyl. For in vivo studies, mice were induced to depress by
unpredictable chronic mild stress for 6 weeks. At week 3, the stress-mice were administered
the SSY extract (20, 100, and 500 mg/kg/day, p.o.) and a reference antidepressant, imipramine
(IMP, 20 mg/kg/day, p.o.) for 6 weeks. A behavioral test was started in week 5. The FST and
TST were performed to evaluate depressive-like behavioral. Results: The SSY inhibited
MAO-A and MAO-B activity with IC50 values of 49.72 ± 9.42 and 329.23 ± 50.80 μg/ml,
respectively. Moreover, the SSY and IMP significantly decreased the immobility time
compared with a control group in both TST and FST. Conclusion: The results of this study
suggest that SSY ameliorates depressive-like symptoms, and the possible mechanism may be
its inhibitory effect on MAOs enzymes. |
Publication
Journal Publication
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