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Publication
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| Research Title |
Effect of mineral trioxide aggregate mixed with Thai propolis extract on matrix metalloproteinase-2 expression in inflamed human dental pulp cells |
| Date of Distribution |
8 November 2023 |
| Conference |
| Title of the Conference |
20th International Scientific Conference of the Dental Faculty Consortium of Thailand (DFCT) "Moving Together Towards the New Dental Era" |
| Organiser |
Mahidol University, Faculty of Dentistry |
| Conference Place |
Royal Cliff Hotels Group and Peach Exhibition Hall |
| Province/State |
Pattaya, Thailand |
| Conference Date |
6 November 2023 |
| To |
8 November 2023 |
| Proceeding Paper |
| Volume |
Vol.43 (2023) |
| Issue |
Suppl October |
| Page |
S57-S66 |
| Editors/edition/publisher |
Assoc.Prof.Dr. Suchaya Pornprasertsuk-Damrongsri |
| Abstract |
Objective: To investigate the inhibition activity of mineral trioxide aggregate (ProRoot®MTA) mixed with Thai propolis extract on the expression of Matrix Metalloproteinase-2 (MMP-2) in IL-1β-stimulated human dental pulp cells (HDPCs), compared to calcium hydroxide (Dycal®), a commonly used dental pulp capping material.
Materials and Methods: HDPCs cultured from three freshly extracted intact third molars were incubated with 10 ng/ml of IL-1β to induce MMP-2 activity. Concentrations of Thai propolis extract (0.25-1.50 mg/ml) were evaluated with PrestoBlue cytotoxic assay. ProRoot®MTA mixed with distilled water, ProRoot®MTA mixed with non-toxic Thai propolis extract, non-toxic Thai propolis extract, and DyCal® were used to treat IL-1β-stimulated HDPCs in vitro. After 24 h of incubation, culture supernatants were collected and evaluated for MMP-2 expression using gelatin zymography.
Results: Thai propolis extract 0.25, 0.50, and 0.75 mg/ml were not toxic to inflamed pulp cells at 24, 48, and 72 h. MMP-2 expression was upregulated in IL-1β–challenged HDPCs. Pro-form of MMP-2 was significantly reduced when IL-1β–stimulated HDPCs were incubated with 0.75 mg/ml Thai propolis extract and Dycal®, lower than MTA mixed with propolis and untreated control. In addition, Thai propolis extract significantly decreased the levels of active MMP-2 than Dycal®. However, the MMP-2 levels were not statistically different among MTA mixed with distilled water and MTA mixed with the propolis extract.
Conclusion: Thai propolis extract reduced an active form of MMP-2, greater than Dycal®. Although Thai propolis extract and Dycal® provide better MMP-2 reduction than MTA groups, Thai propolis extract mixed with MTA showed an insignificant effect on MMP-2 reduction in IL-1β – stimulated HDPCs. |
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| Peer Review Status |
มีผู้ประเมินอิสระ |
| Level of Conference |
ชาติ |
| Type of Proceeding |
Full paper |
| Type of Presentation |
Oral |
| Part of thesis |
true |
| ใช้สำหรับสำเร็จการศึกษา |
ไม่เป็น |
| Presentation awarding |
false |
| Attach file |
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| Citation |
0
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Journal Publication
ไทย