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Publication
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| Research Title |
Impact of Chlorpyrifos on Pycnoporus sanguineus (NUOL-PKK02) and on its possible antagonistic activity against Fusarium wilt of banana under In vitro conditions |
| Date of Distribution |
4 March 2023 |
| Conference |
| Title of the Conference |
The 14th International Conference on Environmental and Rural Development |
| Organiser |
The 14th ICERD Organizing Committee Chair RUA Director of Research and Extension Phnom Penh, Cambodia |
| Conference Place |
Angkor Paradise Hotel |
| Province/State |
Siem Reap in Cambodia |
| Conference Date |
3 March 2023 |
| To |
5 March 2023 |
| Proceeding Paper |
| Volume |
2566 |
| Issue |
14 |
| Page |
p 140 |
| Editors/edition/publisher |
The 14th ICERD Organizing Committee Chair RUA Director of Research and Extension Phnom Penh, Cambodia |
| Abstract |
Chlopyrifos is the main pesticide that has been used in a banana plantation in Laos for controlling pests. The assay was conducted to evaluate the effect of Chlorpyrifos on P. sanguineus mycelia growth and on the potential biological agents in controlling Fusarium wilt of bananas. In the compatibility test were four different concentrations varying from 0, 500, 750, and 1,000 µg/mL. While for the antagonistic activity study, treated P. sanguineus was tested against F. oxysporum f.sp. cubense causal agent of Fusarium wilt of banana disease by using the Bi-culture technique and In vitro bioassay of three crude extracts of P. sanguineus. Significant differences were observed between control, 500, 750, and 1000 µg/mL concentrations of Chlopyrifos used in the inhibition of mycelial growth of P. sanguineus at 72, 75, and 75 % respectively. It shows that P. sanguineus has the ability to tolerate or compatibility with Chlopyrifos as well. Observation from the Bi-culture test found that P. sanguenues inhibited the mycelia growth of F. oxysporum at 53.40 % and 82.38% of conidia production. In bioassay on crude Hexane, Ethyl acetate and Methanol extraction of P. sanguineus inhibited the pathogen as F. oxysporum at 80% of crude Hexane followed by Ethyl acetate and Methanol at 64 and 50%. Regarding from the results of the study indicated the potential for the development of this Lao strain P. sanguineus (NUOL-PKK02) as biological control agent to control Fusarium wilt of bananas and will be the alternative way for farmers to use biopesticide instead of the application of chemical pesticides. It will enable the choice of combining P. sanguenues (NUOL-PPK02) with chemical pesticides for further bioremediation investigation.
Keywords: Antagonistic fungi, bioremediation, P. sanguenues (NUOL-PPK02), compatibility Fusarium oxysporum f.sp.cubense, and Chlopyrifos. |
| Author |
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| Peer Review Status |
มีผู้ประเมินอิสระ |
| Level of Conference |
นานาชาติ |
| Type of Proceeding |
Abstract |
| Type of Presentation |
Poster |
| Part of thesis |
true |
| ใช้สำหรับสำเร็จการศึกษา |
ไม่เป็น |
| Presentation awarding |
false |
| Attach file |
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| Citation |
0
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Journal Publication
ไทย