|
|
Publication
|
| Title of Article |
Effects of Thanaka (Hesperethusa crenulata) Stem Bark Extract on Collagen Activation and Anti-Melanogenesis for Cosmetic Applications |
| Date of Acceptance |
2 January 2024 |
| Journal |
| Title of Journal |
Tropical Journal of Natural Product Research |
| Standard |
OTHER (at https://www.tjnpr.org) |
| Institute of Journal |
Official Journal of Natural Product Research Group |
| ISBN/ISSN |
|
| Volume |
2024 |
| Issue |
1 |
| Month |
February |
| Year of Publication |
2024 |
| Page |
|
| Abstract |
Thanaka (Hesperethusa crenulata) stem bark has been utilized as natural cosmetics, particularly
in Myanmar since a thousand years ago. However, information on bioactive compounds is still
limited. This research aimed to quantify the marmesin levels in the extracts of Thanaka stem bark
using High-Performance Liquid Chromatography (HPLC). Thanaka stem bark samples were
obtained from diverse markets in Myaing Township, Magway Region (S1), Monywa Township
(S2), and Shwebo Township (S3) from Sagaing Region. Moreover, antioxidant activity,
cytotoxicity, collagen production, and anti-melanogenic effects were investigated. The marmesin
content in Thanaka stem bark extracts derived from S1, S2, and S3 were 12.86 ± 0.48, 1.38 ± 0.09,
and 7.61 ± 0.68 mg/g dry extract, respectively. The results of Pearson’s correlation coefficient
revealed a significant relationship between antioxidant activity and total phenolic content. S1
exhibited the most favorable IC50 values for DPPH and ABTS which were 56.43 ± 1.49 µg/mL,
and 14.04 ± 0.05 µg/mL, respectively. Additionally, it demonstrated a corresponding FRAP value
of 337.62 ± 11.00 mM in terms of Ferrous sulfate equivalent per gram dry extract. Cell assays
revealed no toxicity in immortalized human epidermal keratinocytes (HaCaT cells) treated with
extracts at concentrations below 1000 µg/mL. Moreover, human foreskin fibroblasts (HFF-1 cells)
exhibited enhancement in collagen production without any observed toxicity. Furthermore,
Thanaka stem bark extracts could reduce melanin content and provide moderate tyrosinase
inhibition in murine melanoma (B16F10) cells without toxicity. In conclusion, Thanaka stem bark
extract demonstrates promising biological activities for development as a multifunctional skin
care product. |
| Keyword |
Thanaka stem bark, marmesin, keratinocytes, human fibroblasts, melanocytes, collagen production |
| Author |
|
| Reviewing Status |
มีผู้ประเมินอิสระ |
| Status |
ตีพิมพ์แล้ว |
| Level of Publication |
นานาชาติ |
| citation |
true |
| Part of thesis |
true |
| Attach file |
|
| Citation |
0
|
|
|
|
|
|
|
|
|
Journal Publication
ไทย