| Research Title |
Genotypic identification of clinical multidrug-resistant
Klebsiella pneumoniae isolates by whole genome
sequencing (WGS) |
| Date of Distribution |
8 September 2023 |
| Conference |
| Title of the Conference |
Srinagarind Journal Hospital |
| Organiser |
Faculty of Medicine, Khon Kaen University |
| Conference Place |
Faculty of Medicine, Khon Kaen University |
| Province/State |
Khon Kaen |
| Conference Date |
7 September 2023 |
| To |
8 September 2023 |
| Proceeding Paper |
| Volume |
38 |
| Issue |
1 |
| Page |
23-28 |
| Editors/edition/publisher |
Srinagarind Journal Hospital |
| Abstract |
Background and objective: Klebsiella pneumoniae is a common multidrug resistant gram-negative
bacterium basically causing majority of nosocomial infections. Colistin resistant K. pneumoniae has
been posing a matter of a great concern because it is a resort drug treating gram-negative infection.
The aim of this study was to determine genotypic identification of clinical isolates K. pneumoniae.
Methods: Antibiotic susceptibility was determined by broth microdilution method. Whole genome
sequencing was performed on nineteen K. pneumoniae isolates. Three different computational
tools were used for species identification including Kraken2, 16s rRNA, and MLST.
Results: Based on phenotypically, nine of them are resistance to colistin while the other ten are
sensitive to colistin. We found two isolates S9 and S29 that identified as other species rather than
K. pneumoniae. Based on Kraken2 and 16s rRNA, S9 and S29 were identified as K. quasipneumoniae. Unfortunately, we found a non-concordant result between these analysis tools in one isolate.
With Kraken2 and 16s rRNA results, S9 isolate identified as K. quasipneumoniae while MLST identified as K. pneumoniae. Based on phylogenetic analysis, S9 and S29 is actually K. quasipneumoniae
since it is misidentified by MLST and other laboratory methods. Four of nine colistin-resistant
K. pneumoniae isolates (44.44%) were ST147 which is an antimicrobial-resistant high risk
K. pneumoniae.
Conclusions: These results suggested the limitation in routine laboratory methods in identifying K.
pneumoniae and other phylogroups. At the same time, genotyping identification using WGS-based
data highlights it potential in discrimination of these phylogroups of K. pneumoniae. The emerging
of antimicrobial-resistant high-risk K. pneumoniae distribution should be concerned. |
| Author |
|
| Peer Review Status |
มีผู้ประเมินอิสระ |
| Level of Conference |
ชาติ |
| Type of Proceeding |
Full paper |
| Type of Presentation |
Poster |
| Part of thesis |
true |
| Presentation awarding |
false |
| Attach file |
|
| Citation |
0
|
|
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Publication
Journal Publication
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