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Publication
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| Title of Article |
Evaluation of molecular inhibitors of Loop Mediated Isothermal Amplification (LAMP) |
| Date of Acceptance |
11 March 2024 |
| Journal |
| Title of Journal |
Scientific Reports |
| Standard |
SCOPUS |
| Institute of Journal |
Nature Portfolio |
| ISBN/ISSN |
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| Volume |
2024 |
| Issue |
14 |
| Month |
March |
| Year of Publication |
2024 |
| Page |
5916 |
| Abstract |
Loop-mediated isothermal amplification (LAMP) is a cost-effective and easy-to-perform assay that
enables the direct detection of DNA. Its use in point-of-care diagnostic tests is growing, while it has
the potential to be used in presumptive on-the-field forensic tests. Samples are often collected from
complex matrices that contain high levels of contaminants. Herein, we evaluate the effect of seven
common DNA amplification inhibitors on LAMP – bile salts, calcium chloride, hematin, humic acid,
immunoglobulin G, tannic acid and urea. We study the effect of each inhibitor individually in real-time
detection systems coupled with end-point measurements to delineate their inhibitory effects from
the matrix in which they may be found. Our studies show LAMP inhibitors generally delay the onset
of amplicon formation and quench fluorescence at similar or higher concentrations compared to PCR,
but that end-point measurements of LAMP amplicons are unaffected. This is important as LAMP
amplicons can be detected in non-fluorometric ways thus contributing to the assertions that LAMP is
more robust to inhibitors than PCR. |
| Keyword |
Loop-mediated isothermal amplification, Human DNA, Inhibitors, Forensics |
| Author |
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| Reviewing Status |
ไม่มีผู้ประเมินอิสระ |
| Status |
ตีพิมพ์แล้ว |
| Level of Publication |
นานาชาติ |
| citation |
true |
| Part of thesis |
true |
| Attach file |
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| Citation |
0
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Journal Publication
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