| Abstract |
Purpose This study aims to compare the fecal metabolome in post pull-through HD with and without HAEC patients and
healthy young children using nuclear magnetic resonance (NMR) spectroscopy.
Methods Fresh fecal samples were collected from children under 5 years of age in both post-pull-through HD patients and
healthy Thai children. A total of 20 fecal samples were then analyzed using NMR spectroscopy.
Results Thirty-four metabolites identifed among HD and healthy children younger than 5 years were compared. HD samples
demonstrated a signifcant decrease in acetoin, phenylacetylglutamine, and N-acetylornithine (corrected p value=0.01, 0.04,
and 0.004, respectively). Succinate and xylose signifcantly decreased in HD with HAEC group compared to HD without
HAEC group (corrected p value=0.04 and 0.02, respectively). Moreover, glutamine and glutamate metabolism, and alanine,
aspartate, and glutamate metabolism were the signifcant pathways involved, with pathway impact 0.42 and 0.50, respectively
(corrected p value=0.02 and 0.04, respectively).
Conclusion Diferences in class, quantity, and metabolism of protein and other metabolites in young children with HD after
pull-through operation were identifed. Most of the associated metabolic pathways were correlated with the amino acids
metabolism, which is required to maintain intestinal integrity and function. |
Publication
Journal Publication
ไทย