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Publication
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| Research Title |
Exploring the Antioxidant Potential of the Suk Sai-Yad Formula to Mitigating
Unpredictable Chronic Mild Stress-Induced Cognitive Impairment
via Keap1-Nrf2 Pathways |
| Date of Distribution |
19 March 2024 |
| Conference |
| Title of the Conference |
39th International Annual Meeting in Pharmaceutical Sciences (IAMPS39) |
| Organiser |
Faculty of Pharmaceutical Sciences, Chulalongkorn University |
| Conference Place |
Amari Bangkok Hotel (Amari Watergate) |
| Province/State |
Bangkok |
| Conference Date |
19 March 2024 |
| To |
20 March 2024 |
| Proceeding Paper |
| Volume |
39 |
| Issue |
2 |
| Page |
70 |
| Editors/edition/publisher |
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| Abstract |
Suk Sai-Yad (SSY), a traditional Thai herbal formula, is employed clinically at Chao Phraya
Abhaibhubejhr Hospital, Prachinburi for addressing anorexia and insomnia symptoms. The study
aimed to systematically assess the potential therapeutic effects of SSY on cognitive impairment in
unpredictable chronic mild stress (UCMS) mouse. The study explored the therapeutic effects and
potential mechanisms of SSY against UCMS-induced cognitive impairment through in vivo
assessments using the novel object recognition test (NORT; for recognition memory), Y-maze (for
short-term spatial memory), and Morris water maze (MWM; for long-term spatial memory) with a probe
test (for reference memory). Ex vivo evaluations including lipid peroxidation, superoxide dismutase
(SOD), and catalase (CAT) enzymes activities in hippocampus and frontal cortex were also
investigated in order to clarify the underlying mechanisms. Furthermore, the mRNA expression of
antioxidant enzyme-related gene such as Kelch-like ECH-associated protein 1 (Keap1), nuclear factor
erythroid 2-related factor 2 (Nrf2), heme oxygenase-1 (HO-1), and NAD(P)H quinone dehydrogenase
1 (NQO1), were examined. A Liquid chromatograph tandem mass spectrometer (LC-MS/MS) were
used to further identify the active compounds in SSY. Animal experiments demonstrated that SSY
effectively mitigated cognitive impairment in NORT, Y-maze, and MWM tests. Ex vivo experiments
indicated reduced lipid peroxidation, increased SOD and CAT enzymes, and elevated mRNA
expression of Nrf2, HO-1, and NQO1, alongside the inhibition of Keap1 in antioxidant enzyme
pathways. LC-MS/MS analysis revealed active compounds in SSY, encompassing cannabidiol (CBD),
delta-8-Tetrahydrocannabinol (delta-8-THC), delta-9-tetrahydrocannabinol (delta-9-THC), and delta-9-
tetrahydrocannabinol acid A (THCA-A). In conclusion, this study exhibited the substantial therapeutic
potential of SSY formula against UCMS-induced cognitive impairment in mouse model. The molecular
mechanism is mediated through improvement of antioxidant enzyme function, notably the Keap1-Nrf2
signaling pathway and reduction of brain oxidative damage in both hippocampus and frontal cortex.
70 |
| Author |
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| Peer Review Status |
มีผู้ประเมินอิสระ |
| Level of Conference |
นานาชาติ |
| Type of Proceeding |
Abstract |
| Type of Presentation |
Poster |
| Part of thesis |
true |
| Presentation awarding |
false |
| Attach file |
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| Citation |
0
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Journal Publication
ไทย