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Publication
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| Research Title |
Chronic Stress Induced the Expression of Testicular
Caspases and Low Sperm Quality in Mice Induced with
Dexamethasone |
| Date of Distribution |
16 May 2024 |
| Conference |
| Title of the Conference |
7thIASCBC & 46thAAT Annual Conference |
| Organiser |
The Anatomy Association of Thailand |
| Conference Place |
Long Beach Garden Hotel & Spa, Pattaya, Chonburi, Thailand |
| Province/State |
Chonburi, Thailand |
| Conference Date |
14 May 2024 |
| To |
17 May 2024 |
| Proceeding Paper |
| Volume |
2024 |
| Issue |
- |
| Page |
57 |
| Editors/edition/publisher |
- |
| Abstract |
Chronic stress (CS) is a cause of male sub-fertility or infertility, as a consequence of declined testosterone and poor semen quality. CS and glucocorticoid administrations previously showed germ cell apoptosis in rat testes. However, the mechanism by which dexamethasone (DEX)-induced CS affects low sperm quality is still unknown. Therefore, this study aimed to investigate the effect of DEX induced CS on sperm quality using CASA analysis and the expression of apoptotic proteins in testis of adult mice. Male institute of cancer research (ICR) mice were divided into control and DEX induced CS groups. The control mice were injected with sodium phosphate (Na3PO4) while DEX induced CS mice were injected with 4 mg/kg BW of DEX diluted in Na3PO4 for 21 consecutive days. The stress tests (sucrose preference, tail suspension, and forced swimming tests) were used to estimate mice CS behaviors involved with DEX. The epididymal sperm qualities (sperm concentration, motility, morphology,
viability, and acrosome status) were investigated. Testicular histopathology stained with hematoxylin and eosin was observed. The apoptotic protein expressions (Hsp70, caspase3, and caspase9) in testicular lysate were also determined. The results revealed
that mice induced with DEX were significantly increased the immobility and decreased the total sucrose intake. DEX significantly decreased the sperm quality parameters. The testicular damages and decrease of epididymal sperm mass were obviously found in DEX group. Moreover, the expressions of testicular caspase 3 and 9 in DEX group seemed to be increased as compared to control. In conclusion, the CS may induce poor sperm quality which associated with testicular apoptotic pathway in mice. |
| Author |
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| Peer Review Status |
มีผู้ประเมินอิสระ |
| Level of Conference |
นานาชาติ |
| Type of Proceeding |
Abstract |
| Type of Presentation |
Oral |
| Part of thesis |
true |
| Presentation awarding |
false |
| Attach file |
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| Citation |
0
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Journal Publication
ไทย