| Abstract |
Cholangiocarcinoma (CCA) is an aggressive malignancy due to a difficult diagnosis,
limited treatment options, and chemotherapy resistance. New therapeutic strategies are
necessary to improve CCA patients' survival and quality of life. Isothiocyanates (ITCs) are
phytochemical compounds in cruciferous vegetables like broccoli, cabbage, and Brussels
sprouts. These compounds gained interest owing to their potential health benefits. ITCs
display potent anticancer, antioxidant, and anti-inflammatory properties, inhibiting tumor
progression and metastasis. Unfortunately, limited information is available regarding the role
of ITC in the CCA's anticancer effect. The present study investigated the potential impact of
three ITCs (sulforaphane, sulforaphane, and iberin) on the growth of the CCA cell line
(KKU-452). Cell viability and cell cycle analysis were evaluated by sulforhodamine-B assay
(SRB) and propidium iodide (PI) staining, respectively. The results showed that ITCs
decreased cell viability in KKU-452 cells in a dose- and time-dependent manner. IC50 of
sulforaphane, sulforaphene, and iberin were 18.232.06, 17.014.77, and 23.635.02 mM,
respectively, after 24 h treatment, and 11.450.8, 10.840.95 and 13.601.11 mM, respectively,
after 48 h treatment. In addition, ITCs induced cell cycle arrest at G1 and G2/M phases at
12 h and 24 h. Additional experiments are necessary to illustrate the potential of ITCs against
CCA and to understand the underlying mechanisms. |
Publication
Journal Publication
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