| Research Title |
Developing a Split Aptamer-based Biosensor for the detection of Atrazine Herbicide |
| Date of Distribution |
6 June 2024 |
| Conference |
| Title of the Conference |
International Cnference on Integrated Science Biosensor and Bioinnovation for Sustainability and Forensic Science 2024 |
| Organiser |
คณะวิทยาศาสตร์ มหาวิทยาลัยขอนแก่น |
| Conference Place |
อาคารวิทยภาส คณะวิทยาศาสตร์ มหาวิทยาลัยขอนแก่น |
| Province/State |
ขอนแก่น |
| Conference Date |
6 June 2024 |
| To |
7 June 2024 |
| Proceeding Paper |
| Volume |
1 |
| Issue |
1 |
| Page |
177-183 |
| Editors/edition/publisher |
|
| Abstract |
Thailand cultivates major crops such as sugar cane, corn and millet that play a pivotal role in the country's economy and food security. Pesticides are widely used to boost crop yield, however the presence of residual amounts of these compounds in plants and soil pose a significant risk to human health, animal welfare and the environment. One example is atrazine, a compound that is banned in Europe but still used in Thailand and who’s maximum exposure limit is 0.003 mg/L (US Environmental Protection Agency). To effectively monitor its use, there is a demand for methods to detect atrazine in an agricultural setting i.e. rapid on-site detection is crucial. Current methods depend on either: 1) non-specific detection using enzyme acetylcholinesterase, 2) expensive to produce protein antibodies, or 3) cheap DNA aptamers that have practical limitations. Unlike most DNA aptamers, existing atrazine aptamers require sophisticated laboratory techniques such as isothermal titration calorimetry for sensitive detection with simpler fluorescence-based assays having far worse detection limits. In this work, the design of a split-aptamer system to overcome these limitations is explored. A range of oligonucleotide designs are screened in a range of buffer conditions using circular dichroism and fluorescence melting to identify the most promising candidates. The approach involves dividing a known aptamer sequence into two segments. In the absence of atrazine, the split aptamer segments should remain spatially separated and/or interact weakly with each other. In the presence of atrazine, the two strands should undergo conformational changes and assemble to form a strong and stable complex with atrazine. The ultimate goal is to exploit this significant change in structure to improve our ability to detect atrazine binding using simple techniques such as fluorophore-quencher labelling of the two strands to give a fluorescence turn-on/off system that works on-the-field. |
| Author |
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| Peer Review Status |
มีผู้ประเมินอิสระ |
| Level of Conference |
นานาชาติ |
| Type of Proceeding |
Abstract |
| Type of Presentation |
Poster |
| Part of thesis |
true |
| Presentation awarding |
true |
| Award Title |
1st Bronze Poster Presentation Awards |
| Type of award |
รางวัลด้านสิ่งแวดล้อม |
| Organiser |
มหาวิทยาลัยขอนแก่น |
| Date of awarding |
6 มิถุนายน 2567 |
| Attach file |
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| Citation |
0
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Publication
Journal Publication
ไทย