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Publication
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| Research Title |
Protein production of recombinant mutated
hyaluronidase from wasp venom (Vespa
tropica) using yeast expression system |
| Date of Distribution |
15 November 2022 |
| Conference |
| Title of the Conference |
Pichia 2022 conference |
| Organiser |
esib - the European Summit of Industrial Biotechnology |
| Conference Place |
Messe Graz, Messeplatz 1, 8010 Graz, Austria |
| Province/State |
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| Conference Date |
14 November 2022 |
| To |
16 November 2022 |
| Proceeding Paper |
| Volume |
1 |
| Issue |
1 |
| Page |
284 |
| Editors/edition/publisher |
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| Abstract |
Recombinant mutated hyaluronidase (rmVesT2a) protein is the protein that can bind hyaluronic acid without hydrolysable activity. The aim of this study is the production of rmVesT2a protein from wasp venom (Vespa tropica) using a yeast expression system. For this study, the hyaluronidase gene (VesT2a gene; wild type) is cloned from wasp venom. After that, VesT2a gene is mutated at the catalytic site (D107N and E109Q) using the site-directed mutagenesis technique. Next, mVesT2a gene is optimized for codon optimization for Pichia pastoris. Then, this mVesT2a gene is assembled into recipient BB1 and dBB3 using the GoldenPiCS with different promoters which are PAOX1 and PGAP. At the present, we can produce the recombinant protein that MW is approximately 30 kDa by P. pastoris. However, these rmVesT2a proteins must have to be confirmed by some analytical technique such as LC/MS-MS. |
| Author |
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| Peer Review Status |
มีผู้ประเมินอิสระ |
| Level of Conference |
นานาชาติ |
| Type of Proceeding |
Abstract |
| Type of Presentation |
Poster |
| Part of thesis |
true |
| ใช้สำหรับสำเร็จการศึกษา |
ไม่เป็น |
| Presentation awarding |
false |
| Attach file |
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| Citation |
0
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Journal Publication
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