| Research Title |
Developed and Validated LC-MS/MS Method to Determine Quetiapine and Metabolite Plasma Levels in Dementia. |
| Date of Distribution |
26 May 2023 |
| Conference |
| Title of the Conference |
44th Pharmacological and Therapeutic Society of Thailand Meeting |
| Organiser |
สมาคมเภสัชวิทยาแห่งประเทศไทย |
| Conference Place |
โรงแรม แรมแบรนดท์ กรุงเทพฯ |
| Province/State |
กรุงเทพมหานคร |
| Conference Date |
17 December 2024 |
| To |
17 December 2024 |
| Proceeding Paper |
| Volume |
44 |
| Issue |
1 |
| Page |
25 |
| Editors/edition/publisher |
Proceedings of the 44th Annual Meeting of the Pharmacological and Therapeutic Society of Thailand. |
| Abstract |
Background and Objective: Quetiapine ( QTP) is a common atypical antipsychotic drug
used worldwide to treat schizophrenia, acute mania, bipolar depression, and dementia. This
study developed and fully validated to measure QTP and norquetiapine ( NQTP) in human
plasma using a liquid chromatography-tandem mass spectrometry (LC-MS/MS) method by
US-FDA guidelines. Method: One-step liquid-liquid extraction is used a small plasma
volume for extraction with acetonitrile. The analytes were separated on an XBridge BEH C18
column ( 3. 0 x 50 mm, 2. 5 µm) . The mobile phase was 0. 1% formic acid in 10 mM
ammonium formate with 100% acetonitrile (pH 3.0). The chromatogram was performed in
gradient mode at a flow rate of 0.3 ml/min, with a short analytical run time of 6 minutes.
Multiple reaction monitoring (MRM) in positive ion mode was used to quantify QTP, NQTP,
and risperidone as internal standards. Results: The calibration curve was 2.0-600.0 ng/ml
linear range for QTP and NQTP in human plasma. The lower limit of detection for QTP and
NQTP was 2 ng/ml. The coefficient of variation for intra-day and inter-day precision of QTP
was within 3.6-11.2%, and accuracy was within 96.7-111.7%, whereas NQTP precision was
within 5.3-12.9% and accuracy was within 93.2-110.5%. This method assessed the QTP and
NQTP levels of 20 samples in dementia patients. The mean dose of QTP was 216. 25 ±
284.04 mg/d (dose range 25-1100 mg/d). The mean concentrations of QTP and NQTP were
50. 07 ± 53. 68 and 29. 25 ± 35. 47 ng/ ml, respectively. Conclusions: The validated LCMS/MS method was successfully applied to evaluate the QTP and NQTP levels in dementia
patients. |
| Author |
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| Peer Review Status |
มีผู้ประเมินอิสระ |
| Level of Conference |
ชาติ |
| Type of Proceeding |
Abstract |
| Type of Presentation |
Poster |
| Part of thesis |
true |
| ใช้สำหรับสำเร็จการศึกษา |
ไม่เป็น |
| Presentation awarding |
false |
| Attach file |
|
| Citation |
0
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Publication
Journal Publication
ไทย