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Publication
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Research Title |
Electrolyte leakage and fatty acid changing associated with seed germination in accelerated aging sweet pepper seeds |
Date of Distribution |
12 October 2008 |
Conference |
Title of the Conference |
The 13th International Biotechnology Symposium and Exhibition |
Organiser |
Biotechnology for the Sustainability of Human Society |
Conference Place |
Dalian |
Province/State |
China |
Conference Date |
12 October 2008 |
To |
17 October 2008 |
Proceeding Paper |
Volume |
136 |
Issue |
S |
Page |
S149 |
Editors/edition/publisher |
W Zhang, FW Bai, JJ Zhong and SL Yang |
Abstract |
Seed quality is very important in seed germination and impact to seed value, particularly, sweet pepper seed. The percentage of germination and rapid deterioration during storage of sweet pepper seed are major problems that impact on seed quality. In this study, the effects of accelerated aging of sweet pepper seeds in an incubator with 100% relative humidity at 42 ๐C for 0, 5, 10, 15, 20, 25, and 30 days were evaluated the association among seed germination, electrolyte leakage and fatty acid concentration. The results showed that longer aging time decreased the ability of seed germination. The electrolyte leakage such as K+, Na+ Ca2+ and Mg2+ concentration including electrical conductivity (EC) value were determined in 24 hrs soaked seed solution in distilled water. The EC values and K+ in soaked seed solution were increased from 5-30 days of aging seeds. The Na+, Ca2+ and Mg2+ in soaked seed solution were not substantially changed during 0-30 days of aging seeds. The different change in electrolyte leakage concentrations implied the decreasing ability of transporting proteins in cell membrane to control electrolyte in cell that it is not from major damage in lipid bilayer of the cell membrane. The determination of fatty acid concentrations which were palmitic acid, stearic acid, oleic acid linoleic acid and linolenic acid in aging seeds was also studied. The results revealed that all of five fatty acid concentrations were changed in the similar pattern when the aging time was increased. The major change of those fatty acids appeared during 10-20 days of aging time were associated with the ability of seed germination and K+ leakage concentration. The mechanism of lipid peroxidation induced by reactive oxygen species (ROS) causing membrane damage during accelerated aging sweet pepper seeds will be further elucidated. |
Author |
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Peer Review Status |
มีผู้ประเมินอิสระ |
Level of Conference |
นานาชาติ |
Type of Proceeding |
Abstract |
Type of Presentation |
Poster |
Part of thesis |
true |
Presentation awarding |
false |
Attach file |
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Citation |
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