GSMIS

Publication

Journal Publication

Title of Article

Development of SCAR Markers for Species Identification of the Genus Nepenthes (Nepenthaceae)

Date of Acceptance

16 November 2009

Journal

Title of Journal

Pakistan Journal of Biological Sciences

Standard

Institute of Journal

Asian Network for Scientific Information

ISBN/ISSN

1028-8880

Volume

Issue

Month

November

Year of Publication

2009

Page

1455-1461

Abstract

Nepenthes species in Thailand, namely N. mirabilis Druce, N. gracilis Korth., N. smilesii Hemsl., N. ampullaria Jack and N. kampotiana Lecomte, were collected for development of Sequence Characterized Amplified Region (SCAR) marker, a genotype identification tool. Forty Random Amplified Polymorphic DNA (RAPD) primers were screened and three successful primers produced different banding patterns including five candidate species-specific markers. The candidate markers were cloned and sequenced. The marker sequences are 602, 379, 420, 473 and 1,017 bp for N. mirabilis, N. gracilis, N. smilesii, N. ampullaria and N. kampotiana, respectively. Then the sequences were used to design primers for development of a species-specific band being a SCAR marker, including Mir 1, Mir 2 and Mir 3 for N. mirabilis; Gra 1 and Gra 2 for N. gracilis; Smi 1, Smi 2 and Smi 3 for N. smilesii; Amp 1 and Amp 2 for N. ampullaria and Kam 1 and Kam 2 of N. kampotiana. The primers were evaluated with each other Nepenthes species. Finally, species-specific SCAR markers were successfully developed for N. gracilis, N. ampullaria and N. kampotiana. Application of these markers is feasible for identification of Nepenthes species in Thailand.

Keyword

Genotype identifiction, Nepenthes, RAPD, SCAR marker, species identification,

Author

495020050-2	Miss AREE ANUNIWAT [Main Author]
	Science Master's Degree

Reviewing Status

มีผู้ประเมินอิสระ

Status

ตีพิมพ์แล้ว

Level of Publication

นานาชาติ

citation

false

Part of thesis

true

ใช้สำหรับสำเร็จการศึกษา

ไม่เป็น

Attach file

Citation