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Journal Publication
Research Title Inhibition and Mechanism-Based Inactivation of Human CYP3A4 by Phyllanthus amarus  
Date of Distribution 10 May 2009 
Conference
     Title of the Conference The 3rd Asian Pacific Regional ISSX Meeting: Understanding Xenobiotics for Better Drug Development and Therapy 
     Organiser International Society for the Study of Xenobiotics (ISSX) 
     Conference Place The Imperial Queen’s Park Hotel 
     Province/State Bangkok, Thailand 
     Conference Date 10 May 2009 
     To 12 May 2009 
Proceeding Paper
     Volume 41 
     Issue
     Page 39 
     Editors/edition/publisher Jack A. Hinson 
     Abstract Phyllanthus amarus (P. amarus) has long been used as medicinal plant in several countries around the world, including Thailand, for the treatment of jaundice, hepatitis, diabetes and other diseases. It has been reported that alcoholic and aqueous extract of P. amarus could inhibit certain drug metabolizing enzymes both in vivo as well as in vitro. Since CYP3A4 is the major enzyme that responsible for the metabolism of a number of drugs, therefore the inhibitory effects of P. amarus extractions and its 2 major lignans; phyllanthin and hypophyllanthin were determined in the present study using human hepatic microsomal testosterone 6b-hydroxylase as a selective CYP3A4 marker. The ethanolic and aqueous extracts of P. amarus inhibited CYP3A4 activity in a dose-dependent manner with IC50 values of 1.26 ± 2.29 mg/ml and 65.57 ± 4.93 mg/ml, respectively. Furthermore, the soxhlet extractions of P. amarus by hexane, dichloromethane and methanol showed IC50 values of 0.39 ± 0.15 mg/ml, 11.6 ± 4.3 mg/ml and >100 mg/ml, respectively. Phyllanthin and hypophyllanthin which extracted from the hexane fraction showed time–dependent inhibition on CYP3A4. Mechanism-based inhibition kinetics of these lignans were also determined. 
Author
507070016-7 Miss THEERADA TAESOTIKUL [Main Author]
Medicine Doctoral Degree

Peer Review Status มีผู้ประเมินอิสระ 
Level of Conference นานาชาติ 
Type of Proceeding Abstract 
Type of Presentation Poster 
Part of thesis true 
Presentation awarding false 
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