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Publication
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Research Title |
ACCELERATED AGING AND PRIMING OF SWEET PEPPER SEEDS: THE ASSOCIATION BETWEEN SEED GERMINATION AND ELECTROLYTE LEAKAGE |
Date of Distribution |
29 April 2010 |
Conference |
Title of the Conference |
14th International Biotechnology Symposium and Exhibition |
Organiser |
Biotechnology for the Sustainability of Human Society |
Conference Place |
Biotechnology for the Sustainability of Human Society, |
Province/State |
Rimini, Italy |
Conference Date |
29 April 2010 |
To |
29 April 2010 |
Proceeding Paper |
Volume |
1 |
Issue |
1 |
Page |
1 |
Editors/edition/publisher |
k |
Abstract |
ACCELERATED AGING AND PRIMING OF SWEET PEPPER SEEDS: THE ASSOCIATION BETWEEN SEED GERMINATION AND ELECTROLYTE LEAKAGE
Preeya Kaewnaree1, 2, Sukanda Vichitphan2, Preekramol Klanrit2, Boonmee SIRI3 and Kanit Vichitphan2
1 Graduate School, Khon Kaen University, Khon Kaen, 40002, Thailand; 2Department of Biotechnology,Faculty of Technology, Khon Kaen University, 40002, Thailand, kanvic@kku.ac.th;
3 Department of Plant Science and Agricultural Resource, Faculty of Agriculture, Khon Kaen University, Khon Kaen, 40002, Thailand
Vigorous seeds germinate rapidly in a wide range of environmental condition and uniform, healthy seedlings provide good stand establishment in seed quality. Priming improves seed performance rapid and uniform germination, especially under suboptimal condition. In this study, the improvement of seed quality by seed priming at water potential to ~1.5 MPa of polyethylene glycol (PEG 6000) solution for 6 days at 15 ๐C of accelerated aging of sweet pepper seeds with 100% relative humidity at 42 ๐C for 0, 5, 10, 15, 20, 25, and 30 days was evaluated. The results showed that no significant decrease in germination was observed in accelerated aging seeds from 5 to 20 aging days, but thereafter, seed germination progressively decreased on 25 and 30 days of aging. Polyethylene glycol priming solution improved the germination in comparison to the control but no improvement on 25 and 30 days of aging treatment. The percentage of electrolyte leakages (K+, Na+, Ca2+ and Mg2+) of the aging seed increased with the aging treatment time and progressively increased after 25 day of aging. Priming reduced the percentage of electrolyte leakage significantly after accelerated aging, as compared to control. The association between seed germination and electrolyte leakage from aging seeds and priming seeds implied the possibility of the cell membrane deterioration during aging treatment and cell membrane could be repaired during priming process. The lipid peroxidation induced by reactive oxygen species (ROS) causing membrane damage during accelerated aging sweet pepper seeds and membrane improvement from priming process will be further elucidated.
References
[1]McDonald, M.B., 1999. Seed deterioration: physiology, repair and assessment. Seed Science Technology 27: 177-237.
[2]Chiu, K.Y., Chen, C.L., Sung, J.M., 2003. Partial vacuum storage improves the longevity of primed sh-2 sweet corn seeds. Scientia Horticulturae 98: 99-111.
[3] Krissana, B.,Saichol, K. and Wouter, G. 2007. Seed browning of hot peppers during low temperature storage. Posthavest Biology and Technology 45, 358-365.
Keywords: Sweet pepper; Sweet pepper seed; Accelerated aging; Seed priming; Electrolyte leakage; Polyethylene glycol
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Peer Review Status |
มีผู้ประเมินอิสระ |
Level of Conference |
นานาชาติ |
Type of Proceeding |
Abstract |
Type of Presentation |
Oral |
Part of thesis |
true |
Presentation awarding |
false |
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