|
|
Publication
|
| Title of Article |
Differential detection of Brugia malayi and Brugia pahangi by real-time fluorescence resonance energy transfer PCR and its evaluation for diagnosis of B. pahangi-infected dogs |
| Date of Acceptance |
15 December 2009 |
| Journal |
| Title of Journal |
Parasitology Research |
| Standard |
|
| Institute of Journal |
Springer-Verlag Berlin Heidelberg |
| ISBN/ISSN |
|
| Volume |
|
| Issue |
106 |
| Month |
มกราคม |
| Year of Publication |
2010 |
| Page |
621-625 |
| Abstract |
A real-time fluorescence resonance energy transfer
PCR combined with melting curve analysis was
developed for differentiating Brugia malayi and Brugia
pahangi DNA in host blood using one set of primers and
fluorophore-labeled hybridization probes specific for HhaI
repetitive DNA. The differentiation of both species was
based on their melting temperatures (Tm). The mean Tm ±
SD of B. malayi and B. pahangi were 56.18±0.21 and
52.49±0.07, respectively. The method was used for the
molecular detection of B. pahangi in infected dog blood
samples. The diagnostic sensitivity, specificity, accuracy,
and positive and negative predictive values of this method
were 100%. The detected mean difference of the Tm might
allow the simple discrimination of two related species. This
method is fast, sensitive, allows for a high throughput, can
be performed on very small volumes, and has potential for
diagnosis of B. pahangi-infected dogs in endemic areas as
well as for large epidemiological investigations. |
| Keyword |
Brugia malayi , Brugia pahangi, Real-time PCR |
| Author |
|
| Reviewing Status |
มีผู้ประเมินอิสระ |
| Status |
ตีพิมพ์แล้ว |
| Level of Publication |
นานาชาติ |
| citation |
false |
| Part of thesis |
true |
| Attach file |
|
| Citation |
0
|
|
|
|
|
|
|
|
|
Journal Publication
ไทย