2009-2015 ©
             Publication
Journal Publication
Research Title xCELLIgence Real-Time Cytotoxicity Analysis of Lawsone using Human Epidermoid Carcinoma Cells (A431) 
Date of Distribution 2 December 2009 
Conference
     Title of the Conference The 14th All India Congress of Cytology and Genetics & Fogarty International Workshop on Molecular Epidemiology, Environmental Health & Arsenic Exposure Assessment (14th AICCG) 
     Organiser Indian Institute of Chemical Biology and Department of Genetics, University of Calcutta, Kolkata, India 
     Conference Place Indian Institute of Chemical Biology and Department of Genetics, University of Calcutta 
     Province/State Kolkata, India 
     Conference Date 1 December 2009 
     To 4 December 2009 
Proceeding Paper
     Volume 14 
     Issue
     Page 90-91 
     Editors/edition/publisher  
     Abstract Lawsone (2-hydroxy-1,4-naphthoquinone), a red-orange dye presented in leaves of henna plant (Lawsonia inermis), used for cosmetic purposes for skin, hair, and fingernails. This study was aimed to monitor dynamic events of human epidermal carcinoma keratinocytes (A431), when exposed to a series of concentration (1.5 M – 15 mM) of Lawson, based on cell growth and proliferation assay using the xCELLIgence system. The system monitored cell response profile using electrical impedance by measuring real-time cell index. After cell suspensions were seeding into the E-plate 96 well plate (3000 cells/well), A431 were monitored every 15 min until log phase. IC50 from the dose-response curves was found to be about 0.5 mM. This was correlated to MTT end point assay. Real-time and continuous monitoring of keratinocytes which could be suggested cells response mechanism based on dose dependent.  
Author
525150015-2 Miss WEERAYA SUKKHAMDUANG [Main Author]
Pharmaceutical Sciences Master's Degree

Peer Review Status มีผู้ประเมินอิสระ 
Level of Conference นานาชาติ 
Type of Proceeding Abstract 
Type of Presentation Poster 
Part of thesis true 
Presentation awarding false 
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