| Research Title |
Tempporal profiles of copro-antigen and egg DNA of opisthorchis viverrini in experimental opisthorchiasis: Implications for diagnosis and curative chemotherapy |
| Date of Distribution |
6 October 2011 |
| Conference |
| Title of the Conference |
การประชุมวิชาการประจำปี คณะแพทยศาสตร์ ม.ขอนแก่น ครั้งที่ 27 ประจำปี 2554 |
| Organiser |
คณะแพทยศาสตร์ ม.ขอนแก่น |
| Conference Place |
ห้องบรรยาย 1-4 อาคารเตรียมวิทยาศาสตร์คลินิก คณะแพทยศาสตร์ ม.ขอนแก่น |
| Province/State |
ขอนแก่น |
| Conference Date |
4 October 2011 |
| To |
6 December 2011 |
| Proceeding Paper |
| Volume |
27 |
| Issue |
1 |
| Page |
144 |
| Editors/edition/publisher |
|
| Abstract |
Background and Objective: Effective control program of opisthorchiasis requires sensitive and specific diagnostic methods for screening and assessment of curative treatment. The purposes of the study were to compare copro-antigen and egg DNA detection for diagnosis of Opisthorchis viverrini infection in hamster. Performances of these diagnostic methods to assess curative chemotherapy are also evaluated.
Methods: Two groups of hamsters were infected with a dose of 5, 10, 25 or 50 O. viverrini metacercariae/animal (5 hamsters/dose). In group 1, fecal samples of hamsters were collected at weekly intervals from 0-12 wk. Group 2 were treated orally with a single 400 mg/kg of praziquantel at week 6 post infection and fecal samples collected until week12. All fecal samples were examined by a quantitative formalin-ethyl acetate concentration (FECT). The presence of parasite copro-antigen was determined by monoclonal antibody enzyme-linked immunosorbent assay (MAb-ELISA) and egg DNA was detected by polymerase chain reaction (PCR).
Results: By MAb-ELISA, copro antigen were detected in all infected groups of hamsters starting from week 2 post infection while, egg DNA were detected at week 3 and eggs were seen at week 4 post infection. After treatment, no egg or egg DNA was detected at 1 week post-treatment onwards. By contrast, copro-antigen was observed throughout the study period.
Conclusions: PCR method may be useful for both diagnosis and evaluation of treatment while MAb-ELISA is useful for screening only at pre-treatment. The implication of persistence of copro antigen after treatment and its roles in disease development and CCA deserves further study. |
| Author |
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| Peer Review Status |
ไม่มีผู้ประเมินอิสระ |
| Level of Conference |
ชาติ |
| Type of Proceeding |
Abstract |
| Type of Presentation |
Poster |
| Part of thesis |
true |
| ใช้สำหรับสำเร็จการศึกษา |
ไม่เป็น |
| Presentation awarding |
true |
| Award Title |
รางวัลที่ 3 poster presentation |
| Type of award |
รางวัลด้านวิชาการ วิชาชีพ |
| Organiser |
คณะแพทยศาสตร์ ม.ขอนแก่น |
| Date of awarding |
6 ตุลาคม 2554 |
| Attach file |
|
| Citation |
0
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Publication
Journal Publication
ไทย