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Journal Publication
Research Title Synergistic Effects of Acyclovir and Andrographolide Derivative on Drug-Resistant Herpes Simplex Virus Type 1 
Date of Distribution 28 June 2013 
Conference
     Title of the Conference International Conference in Medicine and Public Health (ICMPH) 2013 
     Organiser Faculty of Medicine Siriraj Hospital, Mahidol University Supporting Organization,The Ministry of Public Health, The Rockefeller Foundation 
     Conference Place Faculty of Medicine Siriraj Hospital, Mahidol University 
     Province/State Bangkok, Thailand 
     Conference Date 9 October 2013 
     To 9 October 2013 
Proceeding Paper
     Volume 65,May-June 2013 
     Issue
     Page RS-110 
     Editors/edition/publisher Siriraj Medicine Journal 
     Abstract Acyclovir (ACV) is the common drug for therapy of herpes simplex virus (HSV) infections but acyclovir-resistant HSVs are frequently isolated from immunosuppressed patients. Therefore, the novel antiviral agents are still needed. In our previous report, andrographolide derivative or 3,19-isopropylideneandrographolide (IPAD) has inhibitory effect on HSV replication of both wild type and resistant strain. Therefore, this study aimed to determine synergistic effects of ACV combining with IPAD on drug-resistant HSV-1. Cytotoxicity of IPAD was determined on Vero cell line by MTT assay. Wild type HSV-1 (strains KOS 1-003) and drug-resistant stains consisting of dxpIII (phosphonoacetate- and phosphonoformate-resistant), ACGr4 (acyclovir-resistant with thymidine kinase (TK)-deficient), and dlsptk (acyclovir-resistant with TK deletion) were titrated by plaque assay. Synergistic effects of ACV combining with IPAD on HSV infection was characterized by the combination index (CI) of plaque inhibition. The result showed that non-cytotoxic concentration of IPAD (22.55 µM) completely inhibited infection of both wild types and resistant strains, whereas ACV could not inhibit resistant strain. The combination of ACV and IPAD produced synergistic effect on wide type HSV-1 at lowest concentration of ACV (0.04 μM) and IPAD (12.81 μM). Interestingly, synergistic effect of ACV and IPAD was also found in drug-resistant strains including HSV-1 ACGr4 (22.20 and 17.94 μM), HSV-1 dlsptk (22.20 and 20.50 μM) and HSV-1 dxpIII (22.20 and 12.81 μM). This result suggested that IPAD might be a candidate drug for HSV wild type and acyclovir-resistant HSV-1 therapy. 
Author
545070005-2 Mr. THONGKOON PRIENGPROM [Main Author]
Medicine Master's Degree

Peer Review Status มีผู้ประเมินอิสระ 
Level of Conference นานาชาติ 
Type of Proceeding Abstract 
Type of Presentation Poster 
Part of thesis true 
Presentation awarding false 
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