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Journal Publication
Research Title Epstein-Barr Virus as a Risk Factor of Cervical Cancer by Association with High Risk Human Papillomavirus Episome 
Date of Distribution 26 June 2013 
Conference
     Title of the Conference International Conference in Medicine and Public Health (ICMPH) 2013 
     Organiser Faculty of Medicine Siriraj Hospital, Mahidol University Supporting Organization, The Ministry of Public Health, The Rockefeller Foundation 
     Conference Place Faculty of Medicine Siriraj Hospital, Mahidol University 
     Province/State Bangkok, Thailand 
     Conference Date 24 June 2013 
     To 28 June 2013 
Proceeding Paper
     Volume 65 
     Issue
     Page RS-032 
     Editors/edition/publisher Siriraj medicine journal 
     Abstract Objective(s): Epstein-Barr Virus (EBV) is an etiologic factor in multiple types of cancer that primarily develop in lymphocytes and epithelial cells. To explore the role of EBV in high risk human papillomavirus (HR-HPV) induced carcinogenesis, the correlation of EBV infection with HPV physical status was investigated. Material(s) and Method(s): Cervical tissues consisting of no squamous intraepithelial lesion (noSIL, 38 cases), low grade SIL (LSIL, 85 cases), high grade SIL (HSIL, 81 cases) and cervical carcinoma (CA, 38 cases) were extracted for RNA and DNA using Trizol. DNA was used to detect EBV and HPV by PCR, dot blot hybridization assay and reverse line blot hybridization assay. The physical status of HR-HPV was investigated by amplification of papillomavirus oncogene transcripts assay using the extracted RNA. Results: EBV detection rate in HPV positive cases increased with increasing severity of cervical lesion: noSIL (23.7%), LSIL (29.4%) and HSIL (48.2%). Co-detection of HPV and EBV was significantly higher in HSIL+CA (36.13%) than noSIL+LSIL (13.82%). Physical status could be investigated in 60 HR-HPV cases (12 cases of noSIL+LSIL and 48 cases of HSIL+CA). Only episome was found in noSIL+LSIL. In HSIL+CA, episome was found significantly more often in EBV-HPV co-infections (69.2%) than HPV infection alone (31.8%) whereas integration was found significantly more often in HPV infection alone (68.2%) than EBV-HPV co-infections (30.8%). Conclusion: These results reveal novel information of EBV as a risk factor correlated with HPV episome and suggest that EBV-HPV co-infections in cervix may modulate cervical cancer progression.  
Author
537070011-0 Miss SIRINART AROMSEREE [Main Author]
Medicine Doctoral Degree

Peer Review Status ไม่มีผู้ประเมินอิสระ 
Level of Conference นานาชาติ 
Type of Proceeding Abstract 
Type of Presentation Oral 
Part of thesis true 
Presentation awarding false 
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