| Research Title |
Taxifolin Exerts Cytoprotective Effect by Activation of Nrf2-ARE |
| Date of Distribution |
8 October 2014 |
| Conference |
| Title of the Conference |
การประชุมวิชาการ คณะแพทยศาสตร์ มหาวิทยาลัยขอนแก่น ครั้งที่ 30 ประจำปี 2557 |
| Organiser |
คณะแพทยศาสตร์ มหาวิทยาลัย |
| Conference Place |
คณะแพทยศาสตร์ มหาวิทยาลัย |
| Province/State |
ขอนแก่น |
| Conference Date |
7 October 2014 |
| To |
9 October 2014 |
| Proceeding Paper |
| Volume |
29 |
| Issue |
4 |
| Page |
122-125 |
| Editors/edition/publisher |
Srinagarind Medical Journal |
| Abstract |
Background: Oxidative stress contributes to various deleterious health effects including aging,
cardiovascular disease, chronic inflammation,
neurodegenerative disease and cancer. Cells have
developed adaptive cytoprotective response by up-regulation of the antioxidant defense systems including Nrf2-ARE signaling pathway. Nrf2 (Nuclear factor erythroid 2-related factor 2) protein activated by oxidative stress and electrophiles is contributed to the cytoprotective
effect by induction of antioxidant gene expression through the binding with cis-acting consensus sequence so-called antioxidant response element (ARE). Phytochemicals
are rich in antioxidant activity and some effects are produced by induction of antioxidant genes through Nrf2-ARE.
Methods: Our study have screened for inducers of Nrf2-ARE signaling pathway, using HepG2 cells transiently transfected with plasmid containing ARE-luciferase reporter and plasmid with Renilla-luciferase reporter. The cytotoxicity assay was performed by sulforhodamine B assay.
Results: Taxifolin was able to induce the Nrf2-ARE in ARE-luciferase reporter assay stronger than other tested phytochemicals and did not toxic to HepG2 cells. Taxifolin showed cytoprotective effect in a model of doxorubicin-induced cytotoxicity. When HepG2 cells
were pretreated with taxifolin 18 h, the cytotoxicity of doxorubicin was greatly decreased.
Conclusion: The study showed that inducer of Nrf2-ARE pathway including taxifolin could provide health benefit in protection against oxidative injury.
Key words: Nrf2, Antioxidant, Cytoprotective effect,Doxorubicin, reporter assay |
| Author |
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| Peer Review Status |
มีผู้ประเมินอิสระ |
| Level of Conference |
ชาติ |
| Type of Proceeding |
Full paper |
| Type of Presentation |
Oral |
| Part of thesis |
true |
| Presentation awarding |
false |
| Attach file |
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| Citation |
0
|
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Publication
Journal Publication
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