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Publication
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| Title of Article |
Ultrastructural Localization of Endogenous Exchange Factor for ARF6 in Adrenocortical Cells In Situ of Mice |
| Date of Acceptance |
13 April 2016 |
| Journal |
| Title of Journal |
Acta Histochemica et Cytochemica |
| Standard |
SCOPUS |
| Institute of Journal |
ภาควิชากายวิภาคศาสตร์ คณะแพทยศาสตร์ มหาวิทยาลัยขอนแก่น |
| ISBN/ISSN |
doi: 10.1267/ahc.16008 |
| Volume |
2016 |
| Issue |
49 |
| Month |
June |
| Year of Publication |
2016 |
| Page |
83-87 |
| Abstract |
EFA6 (exchange factor for ARF6) activates Arf6 (ADP ribosylation factor 6) by exchanging
ADP to ATP, and the resulting activated form of Arf6 is involved in the membrane dynamics
and actin re-organization of cells. The present study was attempted to localize EFA6 type D
(EFA6D) in mouse adrenocortical cells in situ whose steroid hormone secretion is generally
considered not to depend on the vesicle-involved regulatory mechanism. In immunoblotting,
an immunoreactive band with the same size as brain EFA6D was detected in homogenates
of adrenal cortical tissues almost free of adrenal capsules and medulla. In immuno-light
microscopy, EFA6D-immunoreactivity was positive in adrenocortical cells and it was often
distinct along the plasmalemma, especially along portions of the cell columns facing the
interstitium. In immuno-electron microscopy, the gold-labeling was more dense in the
peripheral intracellular domains than the central domain of the immunopositive cells. The
labeling was deposited on the plasma membranes in a discontinuous pattern and in
cytoplasmic domains rich in filaments. It was also associated with some, but not all, of
pleiomorphic vesicles and coated pits/vesicles. No labeling was seen in association with lipid
droplets or smooth endoplasmic reticulum. The present finding is in support of the
importance of EFA6D for activation of Arf6 in adrenocortical cells. |
| Keyword |
EFA6D, ultrastructure, membrane dynamics, adrenal cortex, mice |
| Author |
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| Reviewing Status |
มีผู้ประเมินอิสระ |
| Status |
ตีพิมพ์แล้ว |
| Level of Publication |
นานาชาติ |
| citation |
true |
| Part of thesis |
true |
| Attach file |
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| Citation |
0
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Journal Publication
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