บทคัดย่อ |
The silkworm, Bombyx mori, is an economically important insect group for the sericulture or production of silk fiber, processing to silk thread and silk fabric. In addition, the two main proteins from silk cocoon, fibroin and sericin, have been applied in pharmaceuticals and cosmetics such as wound healing, antiwrinkle and anti aging (Yao et al., 2003). However, the major problem in rearing silkworm is pathogenic diseases causing the cocoon crop loss (Saksung and Tawonanukulkit, 1997). One of the major diseases is “grasserie disease” induced by Bombyx mori nucleopolyhedrovirus (BmNPV) infection. Subsequently, many studies have reported proteins involving in antiviral mechanism in silkworm that are Bmlipase-1, serine protease-2, lipase-1 and alkaline trypsin proteins (Ponnuvael et al., 2003; Nakazawa et al., 2004). In Thailand, the classification of mulberry silkworm are divided into three races by their origins that are Thai native, hybrid and foreign silkworm strains. The Thai native silkworms seem more resistant to viruses than the foreign silkworm. However, the levels of resistance to BmNPV infection of silkworm strain have not been reported. Therefore, the prior aim of this study is to investigate the level of potential resistance to BmNPV infection in both the resistant and susceptible larvae strains by comparing target gene expressions using qRT-PCR. |