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Publication
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| Title of Article |
Effect of grape seed extract on skin fibroblasts exposed to UVA light and its photostability in sunscreen formulation |
| Date of Acceptance |
24 August 2020 |
| Journal |
| Title of Journal |
Journal of Cosmetic Dermatology |
| Standard |
SCOPUS |
| Institute of Journal |
Wiley Online Library |
| ISBN/ISSN |
https://doi.org/10.1111/jocd.13711 |
| Volume |
19 |
| Issue |
10 |
| Month |
October |
| Year of Publication |
2020 |
| Page |
1-12 |
| Abstract |
Background: Grape seed extract (GSE) is rich in polyphenolic compounds, particularly
(+)-catechin (C) and (-)-epicatechin (EC). Strong antioxidant activity of these
compounds makes GSE to be value-added to the cosmetics with anti-aging properties.
However, a lack of stability in different environmental conditions makes GSE
challenging for the development of photostable cosmetic sunscreen products.
Aims: To evaluate photoprotective effects of GSE on human dermal fibroblasts irradiated
with UVA light and assess photostability of catechins in cream formulations
containing GSE alone or in combination with octyl methoxycinnamate (OMC).
Methods: MTT assay was used to assess protective effects of GSE on fibroblasts
irradiated with UVA light. A photostability of C and EC in GSE and in cream formulation
containing GSE was investigated using high-performance liquid chromatography
and confirmed by reflection and transmission spectrophotometry using Transpore™
tapes and polymethacrylate (PMMA) plates as substrates.
Results: High UVA doses damaged fibroblast structure and inhibited their growth.
However, GSE increased cell viability and effectively protected them from UVA damage.
Photostability of C and EC was achieved by combination of GSE and OMC that
also improved absorption capacity of UV filter and increased overall efficacy of formulation.
PMMA plates showed better applicability for in vitro photostability testing
of sunscreen formulations. However, despite the instability of Transpore® tape
under heat from UV exposure, it can still be economically a substrate of alternative
choice for screening.
Conclusions: GSE can be used as an effective and sustainable natural resource for
prevention of UV-induced skin damage providing long-term protection against premature
skin aging. |
| Keyword |
anti-aging, fibroblasts, grape seed extract, reflection and transmission UV spectrophotometer, sunscreen, UVA |
| Author |
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| Reviewing Status |
มีผู้ประเมินอิสระ |
| Status |
ตีพิมพ์แล้ว |
| Level of Publication |
นานาชาติ |
| citation |
true |
| Part of thesis |
true |
| Attach file |
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| Citation |
0
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Journal Publication
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