GSMIS

Publication

Journal Publication

Title of Article

Alternative Method for HDL and Exosome Isolation with Small Serum Volumes and Their Characterizations

Date of Acceptance

29 October 2021

Journal

Title of Journal

Separations

Standard

SCOPUS

Institute of Journal

MDPI (Basel, Switzerland)

ISBN/ISSN

Volume

Issue

Month

November

Year of Publication

2021

Page

204

Abstract

High-density lipoprotein (HDL) and exosomes are promising sources of biomarkers. However, the limited sample volume and access to the ultracentrifuge equipment are still an issue during HDL and exosome isolation. This study aimed to isolate HDL and exosomes using an ultracentrifugation-free method with various small serum volumes. HDL was isolated from 200 µL (HDL200) and 500 µL (HDL500) of sera. Three different volumes: 50 µL (Exo50), 100 µL (Exo100), and 250 µL (Exo250) were used for exosome isolation. HDL and exosomes were isolated using commercial kits with the modified method and characterized by multiple approaches. The HDL levels of HDL200 and HDL500 were not significantly different (p > 0.05), with percent recoveries of >90%. HDL200 and HDL500 had the same protein pattern with a biochemical similarity of 99.60 ± 0.10%. The particle sizes of Exo50, Exo100, and Exo250 were in the expected range. All isolated exosomes exhibited a similar protein pattern with a biochemical similarity of >99%. In conclusion, two different serum volumes (200 and 500 µL) and three different serum volumes (50, 100, and 250 µL) can be employed for HDL and exosome isolation, respectively. The possibility of HDL and exosome isolation with small volumes will accelerate biomarker discoveries with various molecular diagnostic approaches.

Keyword

biomarker; exosome; HDL; isolation; small serum volume

Author

617100005-8	Mr. RIAN KA PRAJA [Main Author]
	Graduate School Doctoral Degree

Reviewing Status

มีผู้ประเมินอิสระ

Status

ตีพิมพ์แล้ว

Level of Publication

นานาชาติ

citation

true

Part of thesis

true

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