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Publication
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| Title of Article |
Molecular prevalence and associated risk factors of Cryptosporidium spp. infection in dairy cattle in Khon Kaen, Thailand |
| Date of Acceptance |
6 February 2024 |
| Journal |
| Title of Journal |
Veterinary World |
| Standard |
SCOPUS |
| Institute of Journal |
Veterinary World, Star, Gulshan Park, NH-8A, Chandrapur Road, Wankaner - 363621, Dist. Morbi (Gujarat), India |
| ISBN/ISSN |
2231-0916 |
| Volume |
2024 |
| Issue |
16 |
| Month |
Febuary |
| Year of Publication |
2024 |
| Page |
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| Abstract |
Background and aim: Cryptosporidium spp. is an important parasite in the small intestine of humans and animals, particularly cattle. This study aimed to investigate the molecular prevalence and associated risk factors of Cryptosporidium infection and to identify the species of Cryptosporidium in dairy cattle in 5 districts of Khon Kaen province, Thailand.
Materials and Methods: From July 2020 to October 2021, a total of 296 fecal samples were collected from 3 groups of dairy cattle consisting <3-month-old calves, 3-month-old to 1-year-old calves, and >1-year-old cattle. Cryptosporidium spp. was identified by polymerase chain reaction (PCR) amplifying 18s RNA gene. Both genus specific primers and species primers were used to detect Cryptosporidium confirmed by DNA sequencing. Age, house floor type and water trough type were evaluated as risk factors. All associated risk factor information was analyzed with logistic regression test in the SPSS program.
Results: The PCR results showed that 40 (13.51%) out of 296 samples were positive for Cryptosporidium spp. including C. bovis (57.50%), and C. ryanae (2.50%). There was significant association between Cryptosporidium incidence and age of cattle, and house floor type (P<0.05). NCBI BLAST displayed 99.48-100% nucleotide similarity of each Cryptosporidium spp. isolate with references recorded on GenBank. Phylogenetic analysis demonstrated the close relation of C. bovis and C. ryanae and the reference isolates on GenBank.
Conclusion: The current study confirms that C. bovis and C. ryanae are commonly found in dairy cattle, especially calves, in Khon Kaen, Thailand, and the incident was associated with a number of risk factors. A molecular technique could be influential for species identification. The results from the present study would be useful information for veterinarians and animal owners to realize the existing Cryptosporidium spp. and how to properly manage farms. |
| Keyword |
Bovine cryptosporidiosis, Cryptosporidium, dairy cattle, molecular prevalence, Thailand |
| Author |
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| Reviewing Status |
มีผู้ประเมินอิสระ |
| Status |
ได้รับการตอบรับให้ตีพิมพ์ |
| Level of Publication |
นานาชาติ |
| citation |
false |
| Part of thesis |
true |
| Attach file |
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| Citation |
0
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Journal Publication
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