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Publication
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| Title of Article |
Synergistic effects of acyclovir and 3, 19- isopropylideneandrographolide on herpes simplex virus wild types and drug-resistant strains |
| Date of Acceptance |
23 March 2015 |
| Journal |
| Title of Journal |
BMC Complement Altern Med. |
| Standard |
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| Institute of Journal |
BMC Complement Altern Med. |
| ISBN/ISSN |
DOI 10.1186/s12906-015-0591-x |
| Volume |
2015 |
| Issue |
15:56 |
| Month |
march |
| Year of Publication |
2015 |
| Page |
1-8 |
| Abstract |
Background: An andrographolide analogue, 3, 19-isopropylideneandrographolide (IPAD), exerts an inhibitory effect
on replication of wild-type herpes simplex virus serotype 1 (HSV-1). In this study, we examined the anti-viral activity
of IPAD on HSV wild types (HSV-1 strain KOS and HSV-2 clinical isolate) and HSV-1 drug-resistant strains (DRs). Synergistic
effects of IPAD with acyclovir (ACV) were also evaluated.
Methods: MTT and cytopathic effect (CPE) reduction assays were performed to determine cytotoxicity and
anti-viral activities, respectively. A combination assay was used to determine synergistic effects of IPAD and
ACV. Presence of viral DNA and protein in experimental cells was investigated using the polymerase chain
reaction and western blotting, respectively.
Results: A non-cytotoxic concentration of IPAD (20.50 μM) completely inhibited CPE formation induced by
HSV wild types and HSV-1 DRs after viral entry into the cells. The anti-HSV activities included inhibition of
viral DNA and protein synthesis. The minimum inhibitory concentrations of ACV for HSV wild types and
HSV-1 DRs were 20.20 and 2,220.00 μM, respectively. Combination of ACV with IPAD showed synergistic
effects in inhibition of CPE formation, viral DNA and protein synthesis by HSV wild types as well as HSV-1
DRs. For the synergistic effects on HSV wild types and HSV-1 DRs, the effective concentrations of ACV were
reduced.
Conclusions: These results showed the inhibitory potential of IPAD on HSV wild types and HSV-1 DRs and
suggested that IPAD could be used in combination with ACV for treatment of HSV-1 DRs infections. |
| Keyword |
HSV, Synergistic effects, drug-resistant |
| Author |
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| Reviewing Status |
มีผู้ประเมินอิสระ |
| Status |
ตีพิมพ์แล้ว |
| Level of Publication |
นานาชาติ |
| citation |
false |
| Part of thesis |
true |
| Attach file |
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| Citation |
0
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Journal Publication
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